This LRPflox strain may be useful for studying physiological role of LRP in the uptake of cholesterol-rich lipoproteins from circulation and in maintenance of plasma lipid homeostasis.
Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Lrp1 | low density lipoprotein receptor-related protein 1 |
These LRPflox/flox mutant mice possess a floxed Neo cassette and a single loxP site downstream of exon 2 of the targeted low-density lipoprotein (LDL) receptor-related protein (LRP) 1 gene, Lrp1. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 1 and 2 deleted in the cre-expressing tissue, resulting in inactivation of Lrp1 gene function. This strain may be useful for studying physiological role of LRP in the uptake of cholesterol-rich lipoproteins from circulation and in maintenance of plasma lipid homeostasis.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of the 5' initiation site, and a single loxP site downstream of exon 1 of the low-density lipoprotein (LDL) receptor-related protein (LRP) 1 gene, Lrp1. (Note: the Donating Investigator provided this corrected description of the allele in July 2015. It differs from that found in Nat Biotechnol 14(11):1562-5.) The construct was electroporated into 129S7/SvEvBrd-derived JH1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 2, Joachim Herz |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | LRPflox; lrplox |
Gene Symbol and Name | Lrp1, low density lipoprotein receptor-related protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd |
Chromosome | 10 |
Molecular Note | A loxP-flanked PGK-neomycin selection cassette was inserted 240 bp upstream of the transcription initiation site, and a third loxP site was inserted 3' to exon 1 in intron 2. |
Mutations Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas |
When maintaining a live colony homozygous mice may be bred together.
When using the LRPflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012604 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Lrp1<tm2Her> |
Frozen Mouse Embryo | B6;129S7-Lrp1<tm2Her>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Lrp1<tm2Her>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Lrp1<tm2Her>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Lrp1<tm2Her>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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