B6;129S7-Lrp1^tm2Her/J

Stock number: 012604
Product name: LRP^flox
Research areas: Cardiovascular Research, Metabolism Research, Neurobiology Research, Research Tools

Description

This LRP^flox strain may be useful for studying physiological role of LRP in the uptake of cholesterol-rich lipoproteins from circulation and in maintenance of plasma lipid homeostasis.

Detailed description

These LRP^flox/flox mutant mice possess a floxed Neo cassette and a single loxP site downstream of exon 2 of the targeted low-density lipoprotein (LDL) receptor-related protein (LRP) 1 gene, Lrp1. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 1 and 2 deleted in the cre-expressing tissue, resulting in inactivation of Lrp1 gene function. This strain may be useful for studying physiological role of LRP in the uptake of cholesterol-rich lipoproteins from circulation and in maintenance of plasma lipid homeostasis.

Development

A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of the 5' initiation site, and a single loxP site downstream of exon 1 of the low-density lipoprotein (LDL) receptor-related protein (LRP) 1 gene, Lrp1. (Note: the Donating Investigator provided this corrected description of the allele in July 2015. It differs from that found in Nat Biotechnol 14(11):1562-5.) The construct was electroporated into 129S7/SvEvBrd-derived JH1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Lrp1^tm2Her
Name: targeted mutation 2, Joachim Herz
MGI accession ID: MGI:2180880
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Lrp1
Marker name: low density lipoprotein receptor-related protein 1
Chromosome: 10
Strain of origin: 129S7/SvEvBrd
Molecular note: A loxP-flanked PGK-neomycin selection cassette was inserted 240 bp upstream of the transcription initiation site, and a third loxP site was inserted 3' to exon 1 in intron 2.