B6.129S6-Ptpn11^tm6Bgn/Mmjax

Stock number: 012592
Research areas: Research Tools

Description

These mutant mice possess a loxP-STOP-loxP cassette between exons 2 and 3 and a D61Y point mutation in exon 3 of the protein tyrosine phosphatase, non-receptor type 11 (Ptpn11) gene and may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).

Detailed description

Mice that are homozygous for the targeted mutation are not viable. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).

When bred to a strain expressing Cre recombinase in endothelial cells (Tg(Tek-cre)12Flv), this mutant mouse strain may be useful in studies of the cardiac defects found in Noonan syndrome.

When bred to a strain expressing Cre recombinase in epiblast-derived tissues (Meox2^tm1(cre)Sor), this mutant mouse strain may be useful in studies of cardiac defects.

When bred to a strain expressing tamoxifen-inducible Cre recombinase in most tissues (Tg(CAG-cre/Esr1*)5Amc), this mutant mouse strain may be useful in studies of hematopoiesis.

When bred to a strain expressing interferon-inducible Cre recombinase in most tissues (Tg(Mx1-cre)1Cgn), this mutant mouse strain may be useful in studies of fatal myeloproliferative disorder and juvenile myelomonocytic leukemia (JMML).

When bred to a strain expressing Cre recombinase in neural tube, spinal cord and areas of the brain (Tg(Wnt1-cre)11Rth), this mutant mouse strain may be useful in studies of craniofacial defects.

When bred to a strain expressing Cre recombinase in the early mouse embryo (Tg(EIIa-cre)C5379Lmgd), this mutant mouse strain may be useful in studies of myeloproliferative disorders.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector was designed to place a loxP-neo-loxP-STOP-loxP cassette between exons 2 and 3 and to insert a D61Y point mutation and unique AgeI restriction enzyme site in exon 3 of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for a minimum of 10 generations. Upon arrival, mice were bred to C57LB/6J for at least 1 generation to establish the colony.

Allele

Symbol: Ptpn11^tm6Bgn
Name: targeted mutation 6, Benjamin Neel
MGI accession ID: MGI:3840248
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ptpn11
Marker name: protein tyrosine phosphatase, non-receptor type 11
Chromosome: 5
Strain of origin: 129S6/SvEvTac
Molecular note: A cassette consisting of a loxP site, neo, loxP site, stop signal, and a third loxP site was inserted into intron 2 and a D61Y mutation was inserted into exon 3 via homologous recombination.