B6.129P2(Cg)-Hprt1^{tm43(Ple140-lacZ)Ems}/Mmjax

Stock number: 012577
Strain synonyms: B6.129P2(Cg)-Hprt
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple140-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human NR2E1 gene direct expression of β-galactosidase.

Allele

Symbol: Hprt1^{tm43(Ple140-lacZ)Ems}
Name: targeted mutation 43, Elizabeth M Simpson
MGI accession ID: MGI:4834856
Generation method: Targeted
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in areas of the brain including the hypothalamus and amygdala.
Molecular note: The Ple140-lacZ transgene (pEMS1612) was designed with the 4379 bp Ple140 minipromoter (NR2E1-F; derived from a portion of the promoter of the NR2E1 gene and five upstream putative regulatory elements from the Orphan nuclear receptor NR2E1 (NR2E1) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS4485 has been established.