B6.129P2(Cg)-Hprt1^{tm39(Ple24-lacZ)Ems}/Mmjax

Stock number: 012575
Strain synonyms: B6.129P2(Cg)-Hprt
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple24-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human CCKBR gene direct expression of β-galactosidase.

Allele

Symbol: Hprt1^{tm39(Ple24-lacZ)Ems}
Name: targeted mutation 39, Elizabeth M Simpson
MGI accession ID: MGI:4834848
Generation method: Targeted
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in areas of the brain including the cortex and brain stem.
Molecular note: The Ple24-lacZ transgene (pEMS1495) was designed with the 3813 bp Ple24 minipromoter (CCKBR-C; derived from a portion of the promoter of the CCKBR gene and two downstream putative regulatory elements from the Gastrin/cholecystokinin type B receptor (CCKBR) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS4699 has been established.