This targeted mutation of Zic4 gene displays cerebellar hypoplasia and mild foliation defects. This strain may be useful in the study of cerebellar development and Dandy-Walker malformation.
Kathleen Millen, Seattle Children's Hospital Research Ins
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Zic4 | zinc finger protein of the cerebellum 4 |
Mice that are heterozygous for the targeted mutation are viable, fertile, and normal in size. Heterozygous mice have posterior cerebellar hypoplasia and mild anterior foliation defects. Retinogeniculate projections from the lateral geniculate nucleus exhibit an altered topographic patterning. Homozygous mice are viable and fertile.
This mutant mouse strain may be useful in studies of cerebellar development and Dandy-Walker malformation.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A loxP flanked targeting vector containing neomycin resistance genes was used to replace the coding region of exon 1. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ and maintained as sibling matings. Upon arrival, mice were bred to 129S1/SvImJ for at least 1 generation to establish the colony.
Allele Name | targeted mutation 2, Kathleen J Millen |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Zic4- |
Gene Symbol and Name | Zic4, zinc finger protein of the cerebellum 4 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | A targeting vector was designed to replace the coding region of the first exon with a loxp flanked neo. Transient Cre expression removed the neo in ES cells. |
Mutations Made By | Kathleen Millen, Seattle Children's Hospital Research Ins |
While maintaining a live colony, these mice are bred as homozygotes.
When using the 129-Zic4tm2Kjmi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012560 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Zic4<tm2Kjmi> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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