These FLAG-Lrrk2-WT mice express a wild-type mouse leucine-rich repeat kinase 2 (Lrrk2) gene directed by its endogenous promoter/enhancer regions on a BAC transgene. These mice may be useful for studying normal function of LRRK2 function and the pathogenesis of Parkinson's disease.
Zhenyu Yue, Mount Sinai School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice hemizygous for the BAC FLAG-Lrrk2-Wt transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express a wild-type mouse leucine-rich repeat kinase 2 (Lrrk2) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These BAC FLAG-Lrrk2-Wt mice "overexpress" the wildtype mouse LRRK2 protein in cerebral cortex, striatum, substantia nigra, internal capsule, and hippocampus at an approximately 6-8 fold greater level than endogenous mouse Lrrk2. Contrary to the BAC FLAG-Lrrk2-G2019S Parkinson's disease mice, these mice have enhanced evoked dopamine release in the striatum and exhibit slightly increased motor activities compared to nontransgenic wild-type controls. These mice may be useful for studying normal function of LRRK2 function and the pathogenesis of Parkinson's disease.
Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 240 kb BAC (#RP23-312I9) containing the entire mouse Lrrk2 (leucine-rich repeat kinase 2) gene. This BAC, containing a FLAG-tag downstream from the start codon, was microinjected into fertilized B6C3 F1 oocytes. FLAG-Lrrk2-WT founder line 6 was established and then subsequently maintained by breeding transgenic mice with C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | Lrrk2, leucine-rich repeat kinase 2, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 6, Zhenyu Yue |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | FLAG-LRRK2-WT; LRRK2-WTOE |
Gene Symbol and Name | Tg(Lrrk2)6Yue, transgene insertion 6, Zhenyu Yue |
Gene Synonym(s) | |
Promoter | Lrrk2, leucine-rich repeat kinase 2, mouse, laboratory |
Expressed Gene | Lrrk2, leucine-rich repeat kinase 2, mouse, laboratory |
Strain of Origin | (C57BL/6 x C3H)F1 |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 240 kb BAC (#RP23-312I9) containing the entire mouse Lrrk2 (leucine-rich repeat kinase 2) gene. This BAC contains a FLAG-tag downstream from the start codon. Founder line 6 was established with expression in cerebral cortex, striatum, substantia nigra, internal capsule, and hippocampus at an approximately 6-8 fold greater level than endogenous mouse Lrrk2. |
Mutations Made By | Zhenyu Yue, Mount Sinai School of Medicine |
When maintaining a live colony hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J (Stock No. 000664).
When using the B6.Cg-Tg(Lrrk2)6Yue/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012466 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Lrrk2)6Yue |
Frozen Mouse Embryo | B6.Cg-Tg(Lrrk2)6Yue/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Lrrk2)6Yue/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Lrrk2)6Yue/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Lrrk2)6Yue/J | $3373.50 |
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