B6;129S4-Foxd1^{tm1(GFP/cre)Amc}/J

Stock number: 012463
Product name: FoxD1^GC
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Internal/Organ Research, Research Tools

Description

The Foxd1^{tm1(GFP/cre)Amc} (or FoxD1^GC) allele expresses an eGFPCre fusion protein from the Foxd1 promoter/enhancer elements. These mice may be useful for studying therapeutic strategies directly targeting pericyte differentiation in vivo and may productively impact fibrotic kidney disease.

Detailed description

Heterozygous mice are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The DI states that the strain is homozygous lethal. The FoxD1^GC allele expresses an eGFPCre fusion protein (EGFP and cre fusion protein) from the Foxd1 promoter/enhancer elements. When Foxd1 expression is activated, EGFP immunofluorescence is observed during kidney development in metanephric mesenchyme in cells fated to become stromal cells of the kidney. When FoxD1^GC mice are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Foxd1-expressing cells of the offspring. These mice may be useful for studying therapeutic strategies directly targeting pericyte differentiation in vivo and may productively impact fibrotic kidney disease.

Development

A targeting vector was designed to insert an eGFPCre (GC) fusion protein coding sequence and frt-flanked PGKneobpA selection cassette into the 5' untranslated region of the Forkhead box D1 (Foxd1) targeted gene. Specifically, this targeting vector contained an enhanced green fluorescent protein (EGFP) and a Cre recombinase. This construct was electroporated into F1 hybrid (C57BL/6 x 129S4Sv/Jae)-derived v6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6J females. These FoxD1-GCE (FoxD1^GC) mutant mice were maintained on this mixed C57BL/6J x 129/Sv genetic background. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Foxd1^{tm1(GFP/cre)Amc}
Name: targeted mutation 1, Andrew P McMahon
MGI accession ID: MGI:4359653
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Foxd1
Marker name: forkhead box D1
Chromosome: 13
Strain of origin: (C57BL/6 x 129S4/SvJae)F1
Expressed genes: cre,cre recombinase,bacteriophage P1; GFP,Green Fluorescent Protein,
Site of expression: EGFP immunofluorescence is observed, during kidney development in metanephric mesenchyme in cells fated to become stromal cells of the kidney.
Molecular note: A targeting vector was designed to insert an eGFP/cre (GC) fusion protein coding sequence and frt-flanked PGK-neob-pA selection cassette into the 5' untranslated region of the Forkhead box D1 (Foxd1) targeted gene.