SF1-Cre transgenic mice have Cre recombinase expression detected in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus as well as pituitary, gonad, and adrenal tissue. These mice may be useful for generating tissue specific-targeted mutants for studies of the subset of SF1-positive neurons, obesity, metabolic and energy homeostasis, and appetite control/satiety.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
---|---|
F?+N1F6
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female 4-week |
356.51 Domestic price for breeder pair |
These transgenic mice express Cre recombinase under the direction of the mouse Nr5a1, nuclear receptor subfamily 5, group A, member 1, promoter. The Cre recombinase activity pattern mimics the endogenous gene expression (mRNA) pattern. Cre mediated recombination is detected in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence in the offspring.
A targeting construct containing an FRT site flanked kanamycin resistance cassette and sequence encoding Cre recombinase was inserted into the translational start site of the gene in a BAC. The selection cassette was removed by transient Flp-recombinase expression. The resulting transgene was microinjected into FVB fertilized oocytes. Founder line 7 was subsequently established. The mice were crossed to 129, C57BL/6J and FVB backgrounds. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | Cre mediated recombination is detected in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus as well as pituitary, gonad, and adrenal tissue. |
Allele Name | transgene insertion 7, Bradford B Lowell |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Line S7; SF1Cre; Sf1-cre |
Gene Symbol and Name | Tg(Nr5a1-cre)7Lowl, transgene insertion 7, Bradford B Lowell |
Gene Synonym(s) | |
Promoter | Nr5a1, nuclear receptor subfamily 5, group A, member 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre mediated recombination is detected in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus as well as pituitary, gonad, and adrenal tissue. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | This transgene includes cre recombinase under the control of Nr5a1 regulatory sequences. |
Mutations Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) |
When maintaining a live colony, transgenic mice may be bred to wildtype (noncarrier) mice from the colony or with FVB/NJ inbred mice (Stock No. 001800). The Donating Investigator has not attempted to make the strain homozygous.
When using the Sf1-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #012462 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Nr5a1-cre)7Lowl |
Frozen Mouse Embryo | STOCK Tg(Nr5a1-cre)7Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Nr5a1-cre)7Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Nr5a1-cre)7Lowl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Nr5a1-cre)7Lowl/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.