B6N.129-Ptch1^tm1Hahn/J

Stock number: 012457
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These Ptch^flox mutant mice possess a loxP sites flanking exons 8-9 of the targeted gene. This strain may be useful for studying Hedgehog/Patched signaling, cell-fate determination during embryogenesis, cell growth and differentiation, and development of T- and B-lymphoid lineages, hematopoietic stem cell diversification.

Detailed description

These Ptch^flox mutant mice possess loxP sites flanking exons 8-9 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 8-9 deleted in the cre-expressing tissue. The donating investigator reports that the frt-flanked neo cassette is still present downstream of the floxed exon that the presence of neo does not convey any abnormalities. This strain may be useful for studying Hedgehog/Patched signaling, cell-fate determination during embryogenesis, cell growth and differentiation, and development of T- and B-lymphoid lineages, hematopoietic stem cell diversification.

Development

A targeting vector was designed to insert a loxP site upstream of exon 7, and a second loxP site followed by a frt-flanked neomycin resistance (neo) cassette just downstream of exon 9 of the Patched (Ptch) targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6 females. Mice heterozygous for this Ptch^flox allele were bred together to generate homozygotes. This line was backcrossed to C57BL/6N for 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

Allele

Symbol: Ptch1^tm1Hahn
Name: targeted mutation 1, Heidi Hahn
MGI accession ID: MGI:3764517
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ptch1
Marker name: patched 1
Chromosome: 13
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted into intron 7 and a second loxP site followed by a frt-flanked neo cassette was inserted into intron 9. Insertion was confirmed by Southern blot analysis and PCR genotyping.