These Ptchflox mutant mice possess a loxP sites flanking exons 8-9 of the targeted gene. This strain may be useful for studying Hedgehog/Patched signaling, cell-fate determination during embryogenesis, cell growth and differentiation, and development of T- and B-lymphoid lineages, hematopoietic stem cell diversification.
Heidi Hahn, Institute of Human Genetics
These Ptchflox mutant mice possess loxP sites flanking exons 8-9 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 8-9 deleted in the cre-expressing tissue. The donating investigator reports that the frt-flanked neo cassette is still present downstream of the floxed exon that the presence of neo does not convey any abnormalities. This strain may be useful for studying Hedgehog/Patched signaling, cell-fate determination during embryogenesis, cell growth and differentiation, and development of T- and B-lymphoid lineages, hematopoietic stem cell diversification.
A targeting vector was designed to insert a loxP site upstream of exon 7, and a second loxP site followed by a frt-flanked neomycin resistance (neo) cassette just downstream of exon 9 of the Patched (Ptch) targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6 females. Mice heterozygous for this Ptchflox allele were bred together to generate homozygotes. This line was backcrossed to C57BL/6N for 10 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Heidi Hahn|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ptch1, patched 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A loxP site was inserted into intron 7 and a second loxP site followed by a frt-flanked neo cassette was inserted into intron 9. Insertion was confirmed by Southern blot analysis and PCR genotyping.|
|Mutations Made By|| |
Frauke Nitzki, Institute of Human Genetics
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.129-Ptch1tm1Hahn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012457 in your Materials and Methods section.