C57BL/6N-PRX-B6N #1 mES cells were derived from day 3.5 blastocysts of strain C57BL/6N and expanded on primary mouse embryonic fibroblasts (MEFs) in medium containing 1000U/mL LIF and cultured at 7.5% CO2 in humidified air at 37oC. Pluripotency was confirmed by microinjection of 12-14 cells into BALB/cJ, B6(Cg)-Tyrc-2J, and C57BL/6J blastocysts, as well as immunocytochemistry using antibodies reactive with SSEA-1 and Oct3/4 (see Figures 1 and 2 in the product spec sheet).
 
<a href="http://Jackson.jax.org/rs/444-BUH-304/images/012448C02productspec-P8.pdf">View the mES Cell Product Sheet, Passage 8</a>
 
Produced by <a href="http://www.primogenix.com">Primogenix</a>. Testing for germ line competency, where completed, is documented on the spec sheet for these mES cells. JAX makes no guarantee that this cell line can be used to generate targeted clones or, if targeted clones are created, that they will be germ line competent.

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