Adrbk2 (adrenergic receptor kinase, beta 2; also called GRK3) is the major G protein-coupled receptor kinase family member in olfactory neurons, and mice lacking GRK3 exhibit a deficit in desensitization to olfactants. In homozygous mice, airway smooth muscle cells exhibit supersensitivity to muscarinic agents, and brain neurons exhibit altered kappa opioid receptor-dependent tolerance.
Robert Lefkowitz, Duke University Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Grk3 | G protein-coupled receptor kinase 3 |
Adrbk2 (adrenergic receptor kinase, beta 2; also called GRK3) is the major G protein-coupled receptor kinase family member in olfactory neurons, and mice lacking GRK3 exhibit a deficit in desensitization to olfactants. In homozygous mice, airway smooth muscle cells exhibit supersensitivity to muscarinic agents, and brain neurons exhibit altered kappa opioid receptor-dependent tolerance. Expression is eliminated in olfactory neuroepithelium, brain, and airway smooth muscle cells.
Exons 7 and 8 (encoding part of the protein kinase catalytic domain) were replaced with a PGK-neomycin resistance cassette (in the antisense orientation). The mutation was created in a 129-derived embryonic stem (ES) cell line. The strain was backcrossed more than ten times to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 1, Robert J Lefkowitz |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GRK3 (-) |
Gene Symbol and Name | Grk3, G protein-coupled receptor kinase 3 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 5 |
Molecular Note | Exons 7 and 8 (encoding part of the protein kinase catalytic domain) were replaced with a PGK-neomycin resistance cassette (in the antisense orientation) by homologous recombination. Included in the deleted region was sequence encoding the ATP binding motif of catalytic subdomain I. While transcript was detected by Northern blot analysis of homozygous mutant mice, the absence of protein was verified by Western blot analysis. |
Mutations Made By | Robert Lefkowitz, Duke University Medical Center |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129-Grk3tm1Rjl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012431 in your Materials and Methods section.
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Heterozygous for Adrbk2<tm1Rjl> |
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