NOD.B10Sn-Idd5.2^C57BL/10SnJ Idd5.3^C57BL/10SnJ/7341MrkTacJ

Stock number: 012396
Product name: NOD.B10-Idd5.2/3 R7341
Strain synonyms: NOD.B10<Idd5R7341>, Line 7341, R7341
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of 9 NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344, 012391, 012392, 012393, 012394, and 012395 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Detailed description

This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd5R7341, contains two genomic segments containing Idd5.2 and Idd5.3, from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1. The C57BL/10SnJ genomic segments include Idd5.2 that contains major candidate gene Nramp1 and Idd5.3 containing major candidate gene Ikcf2. Diabetes incidence similar to NOD is not protected in this NOD.B10-Idd5.2, Idd5.3 strain. This strain may be useful for understanding the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.2, Idd5.3 alleles with other Idd alleles.

Development

This NOD/MrkTac congenic strain contains two C57BL/10SnJ (Stock No. 000666) derived genomic segments, introgressed into chromosome 1. The C57BL/10SnJ genomic segments include Idd5.2 and Idd5.3. The Idd5.2 region contains major candidate gene Nramp1 and the Idd5.3 region contains major candidate genes Ikoros family zinc finger 2 protein, Ikcf2, and acyl CoA dehydrogenase, Acadl. This strain was produced by mating published strains 6146 (Stock No. 012394) together with strain 6360 (Hunter, et al 2007). Siblings carrying the C57BL/10SnJ derived segments were intercrossed to make homozygous. NCBI based 5kSNP Chip Data identified no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Allele

Symbol: Idd5.2^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036879
Generation method: QTL
Marker symbol: Idd5.2
Marker name: insulin dependent diabetes susceptibility 5.2
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.

Allele

Symbol: Idd5.3^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3828055
Generation method: QTL
Marker symbol: Idd5.3
Marker name: insulin dependent diabetes susceptibility 5.3
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.