NOD.B10Sn-Idd5.3^C57BL/10SnJ/7345MrkTacJ

Stock number: 012395
Product name: NOD.B10-Idd5.3, R7375
Strain synonyms: NOD.B10<Idd5R7345>, Line 7345, R7345
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of 9 NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344, 012391, 012392, 012393, 012394, and 012396 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Detailed description

This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd5.3R7345, contains a 3.55Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1. The C57BL/10SnJ genomic segment includes Idd5.3 and contains major candidate gene Ikcf2 and Acadl. Diabetes incidence among 7-month old females is reported to be equivalent to published stock 6360 (Hunter, et al. 2007), which is significantly protected with about 69% of the females becoming diabetic, compared with 83% in NOD controls.

This strain may be useful for positional mapping the Idd5.3 region. In addition, this strain is useful for understanding the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.3 alleles with other Iddalleles

Development

This NOD/MrkTac congenic strain contains a C57BL/10SnJ (Stock No. 000666) derived genomic segment, introgressed into chromosome 1. The C57BL/10SnJ genomic segment includes Idd5.3. This region contains 11 annotated genes, including major candidate genes Ikoros Family Zinc Finger 2 protein, Ikcf2, and acyl CoA dehydrogenase, Acadl. This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/10SnJ followed by more than 10 additional crosses to NOD with selection for chromosome 1, Idd5.3 markers. This strain contains a slightly shorter (7Mb) B10Sn congenic interval than published (Hunter, et al. 2007) strain NOD.B10-Idd5.3, line 6360 (7.3Mb). Siblings carrying the C57BL/10SnJ derived segments were intercrossed to make homozygous. NCBI based 5kSNP Chip Data identified no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Allele

Symbol: Idd5.3^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3828055
Generation method: QTL
Marker symbol: Idd5.3
Marker name: insulin dependent diabetes susceptibility 5.3
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.