NOD.B10Sn-Idd5.2^C57BL/10SnJ/6146MrkTacJ

Stock number: 012394
Product name: NOD.B10-Idd5.2, R6146
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of 9 NOD.B10 congenic Idd5 strains (Stock No. 008893, 008894, 004344, 012391, 012392, 012393, 012395, and 012396 that resulted by crossing segments of C57BL/10SnJ derived chromosome 1 into the NOD/MrkTac background. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci; for studying long-range chromosome remodeling and may be useful for identifying diabetes susceptible candidate genes within the Idd5 subloci.

Detailed description

This NOD/MrkTac congenic strain, commonly referred to as NOD.B10-Idd5R6146, contains a 3.85Mb genomic segment from the diabetes resistant strain C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1 and is defined within markers D1Mit532 (73Mb) through D1Mit132 (77Mb). The C57BL/10SnJ genomic segment includes Idd5.2 and contains major candidate gene Nramp1. Diabetes incidence is significantly protected among 7-month old females. Diabetes incidence among 7-month old females is reported to be about 38%, compared with 80% in NOD controls or 62% in NOD.B10-Idd5.1 (Stock No. 012391) (Hunter, et al. 2007).

This strain may be useful for positional mapping the Idd5.2 region. In addition, this strain is useful for understanding the variation in diabetic resistance conferred by Idd loci, in particular the epistatic interactions of Idd5.2 alleles with other Idd alleles.

Development

This NOD/MrkTac congenic strain contains a genomic segment, D1Mit532 (73Mb) through D1Mit132 (77Mb) from C57BL/10SnJ (Stock No. 000666) introgressed into chromosome 1. The C57BL/10SnJ genomic segment includes Idd5.2 and contains major candidate gene Nramp1. This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/10SnJ followed by 17 additional crosses to NOD with selection for chromosome 1, Idd5 markers. Siblings carrying the C57BL/10SnJ derived segments denoted by markers D1Mit532 (73Mb) through D1Mit132 (77Mb) were intercrossed to make homozygous. NCBI based 5kSNP Chip Data identified no C57BL/10SnJ genetic contamination of other chromosomes. The Type 1 Diabetes Resource received this strain in 2010.

Allele

Symbol: Idd5.2^C57BL/10SnJ
Name: C57BL/10SnJ
MGI accession ID: MGI:3036879
Generation method: QTL
Marker symbol: Idd5.2
Marker name: insulin dependent diabetes susceptibility 5.2
Chromosome: 1
Strain of origin: C57BL/10SnJ
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD/MrkTac.