B6CBA-Tg(Myh6-Ppard)HEDpk/J

Stock number: 012383
Research areas: Diabetes and Obesity Research, Internal/Organ Research, Metabolism Research

Description

The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia.

Detailed description

The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of Ppard (peroxisome proliferator activator receptor delta) in these transgenic mice. Hemizygotes have increased myocardial glucose utilization, do not accumulate myocardial lipid, and have normal cardiac function. Expression levels in this "high expression" (HE) line are increased approximately 100-fold over endogenous levels. This strain may be useful in studies of cardiac function and metabolic modulation during diabetes and ischemia.

Development

A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The transgenic construct was microinjected into B6CBAF1 embryos. This mixed genetic background was maintained by the donating laboratory by crossing offspring to B6CBAF1. These mice represent the "high expression" line described in the original reference.

Allele

Symbol: Tg(Myh6-Ppard)HEDpk
Name: transgene insertion HE, Daniel P Kelly
MGI accession ID: MGI:4429481
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Myh6-Ppard)HEDpk
Marker name: transgene insertion HE, Daniel P Kelly
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: Ppard,peroxisome proliferator activator receptor delta,mouse, laboratory
Molecular note: A mouse Ppard cDNA was cloned downstream of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Three lines were created with low (20-fold over levels of the corresponding nuclear receptor), medium (50-fold), and high (100-fold) expression levels (LE, ME, and HE). This is a high expression line.