The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) cardiac muscle-specific promoter drives expression of FLAG-tagged Ppara (peroxisome proliferator activated receptor alpha) in this transgenic strain. Hearts of hemizygotes depend on fatty acid for the generation of energy to a significantly greater extent than do hearts of non-transgenic mice and have a substrate utilization profile similar to that of the diabetic heart. Cardiac dysfunction is exhibited by 4-5 months of age with no imposed stress, or after being placed on a high fat diet. This strain may be useful in studies of metabolic alterations associated with diabetic cardiomyopathy and other forms of lipotoxic cardiomyopathy. This is the "low expressor" line.
Daniel Kelly, Burnham Institute for Medical Research
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) cardiac muscle-specific promoter drives expression of FLAG-tagged Ppara (peroxisome proliferator activated receptor alpha) in this transgenic strain. Expression of Ppara is increased approximately 15-fold as compared to wildtype in this line (404-4). Hearts of hemizygotes depend on fatty acid for the generation of energy to a significantly greater extent than do hearts of non-transgenic mice and have a substrate utilization profile similar to that of the diabetic heart. Cardiac dysfunction is exhibited by 4-5 months of age with no imposed stress, or after being placed on a high fat diet. This strain may be useful in studies of metabolic alterations associated with diabetic cardiomyopathy and other forms of lipotoxic cardiomyopathy.
The mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of mouse Ppara (peroxisome proliferator activated receptor alpha) cDNA carrying a C-terminal FLAG tag. The transgenic construct was introduced to B6CBAF1 hybrid embryos. This strain was backcrossed 8 times to C57BL/6 by the donating laboratory. This is a low expressor line (404-4).
|Expressed Gene||Ppara, peroxisome proliferator activated receptor alpha, mouse, laboratory|
|Site of Expression|
|Allele Name||transgene insertion 404-4, Daniel P Kelly|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Gene Symbol and Name||Tg(Myh6-Ppara)404-4Dpk, transgene insertion 404-4, Daniel P Kelly|
|Promoter||Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine|
|Expressed Gene||Ppara, peroxisome proliferator activated receptor alpha, mouse, laboratory|
|Strain of Origin||(C57BL/6 x CBA/J)F1|
|Mutations Made By|| |
Teresa Leone, Burnham Institute for Medical Research
When maintained as a live colony, hemizygotes may be bred. Integration of the transgene near the agouti allele (associated with the non-agouti gene a on mouse Chromosome 2) may have occurred because many of the transgenic animals are agouti in color despite many backcrosses to C57BL/6 (black).
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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