These mice have the Ple5-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human AMOTL1 gene direct expression of β-galactosidase.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression patterns have not been characterized. |
Allele Name | targeted mutation 66, Elizabeth M Simpson |
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Allele Type | Targeted |
Allele Synonym(s) | AMOTL1-lacZ; Hprttm66(mEMS4645)Ems; Ple5 |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | AMOTL1, angiomotin like 1, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression patterns have not been characterized. |
Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS4645 has been established. |
Molecular Note | The Ple5-lacZ transgene (bEMS90) was designed with the Ple5 MaxiPromoter (AMOTL1-X; derived from the BAC RP11-936P10 that contains the whole Angiomotin like 1 (AMOTL1) gene (along with ~60 kb of 5' and ~10 kb of 3' flanking sequences)) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
When using the STOCK Hprttm66(Ple5-lacZ)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32946 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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