STOCK Hprt1^{tm66(Ple5-lacZ)Ems}/Mmjax

Stock number: 012354
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple5-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human AMOTL1 gene direct expression of β-galactosidase.

Allele

Symbol: Hprt1^{tm66(Ple5-lacZ)Ems}
Name: targeted mutation 66, Elizabeth M Simpson
MGI accession ID: MGI:4834844
Generation method: Targeted
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression patterns have not been characterized.
Molecular note: The Ple5-lacZ transgene (bEMS90) was designed with the Ple5 MaxiPromoter (AMOTL1-X; derived from the BAC RP11-936P10 that contains the whole Angiomotin like 1 (AMOTL1) gene (along with ~60 kb of 5' and ~10 kb of 3' flanking sequences)) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS4645 has been established.