JAX Mouse Strain Datasheet - 012346

B6;129-Terf2ip^tm1.1Tdl/J

Stock No:: 012346

Cryo Recovery

Overview

These Rap1^F mice harbor loxP sites flanking exon 2 (encoding the TRF2 binding domain) of the mouse Rap1 locus (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2 interacting protein). Telomeres serve a dual role in protecting the chromosome ends from degradation/repair activities and in intracellular signaling for regulating cell proliferation. Mammalian telomeres are formed by tandem TTAGGG sequence repeats bound by a specialized complex of six telomere-associated proteins called the shelterin complex. As Rap1 is one of the components of shelterin, these Rap1^F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.

Donating Investigator

Titia de Lange, The Rockefeller University

Genetic overview

Genetic Background	Generation

Terf2ip^tm1.1Tdl

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Terf2ip	telomeric repeat binding factor 2, interacting protein

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Research Applications

Cancer Research
Cell Biology Research
Immunology, Inflammation and Autoimmunity Research
Neurobiology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous for this Rap1^F allele are viable and fertile, with loxP sites flanking exon 2 of the mouse Rap1 gene (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2 interacting protein). When bred to mice that express Cre recombinase, the resulting offspring will have the sequences encoding the TRF2 binding domain deleted in the cre-expressing tissues. As Rap1 is one of the components of shelterin, these Rap1^F mutant mice may be useful in generating conditional mutations for studying the shelterin complex of telomeres, telomere maintenance, chromosomal stability, cancer, and aging.

Development

A targeting vector was designed to insert a frt-flanked neomycin cassette and loxP site upstream of exon 2, and a second loxP site (together with an NdeI site) downstream of exon 2 of the mouse Rap1 locus (also called mRap1, TRF2-interacting factor, Terf2ip, or telomeric repeat binding factor 2, interacting protein). The construct was electroporated into CY2.4 embryonic stem (ES) cells derived from C57BL/6J-Tyr^c-2J (aka B6(Cg)-Tyr^c-2J/J ; Stock No. 000058). Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with albino C57BL/6J-Tyr^c-2J females to establish the colony. Rap1 mutant mice were then bred to FLPe-deleter mice (B6;129 mixed genetic background; derived from Stock No. 003946) to remove the frt-flanked neo cassette. The resulting Rap1^F mice (with loxP site and single frt site remaining upstream of exon 2, and a second loxP site downstream of exon 2) were selected. Black Rap1^F mice were bred together (and the FLPe-expressing transgene was removed) for many generations, and then backcrossed at least one generation to C57BL/6J mice prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. These Rap1^F mice may still be segregating for the Tyr^c-2J mutation.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

Sfeir A; Kabir S; van Overbeek M; Celli GB; de Lange T. 2010. Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Science 327(5973):1657-61. PubMed: 20339076 MGI: J:158884

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Genetics

Terf2ip^tm1.1Tdl

Allele Symbol: Terf2ip^tm1.1Tdl

Allele Name	targeted mutation 1.1, Titia de Lange
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Rap1^F
Gene Symbol and Name	Terf2ip, telomeric repeat binding factor 2, interacting protein
Gene Synonym(s)	DRIP5; RAP1; Rap1
Strain of Origin	B6(Cg)-Tyr
Chromosome	8
Molecular Note	An frt flanked neo cassette with a 3' loxP site was inserted upstream of exon 2 and an additional loxP site was inserted downstream of exon 2. Flp mediated recombination removed the neo cassette leaving exon 2 floxed.
Mutations Made By	Titia de Lange, The Rockefeller University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cancer Research
- Genes Regulating Growth and Proliferation
- Other
  - DNA Repair
Cell Biology Research
- DNA Damage Response
- Genes Regulating Growth and Proliferation
- Signal Transduction
- Transcriptional Regulation
Immunology, Inflammation and Autoimmunity Research
- Intracellular Signaling Molecules
Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
Research Tools
- Cancer Research
- Cell Biology Research
- Cre-lox System
  - loxP-flanked Sequences
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
- Reproductive Biology Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Terf2ip^tm1.1Tdl/Terf2ip⁺
involves: C57BL/6J

no phenotypic analysis

no phenotypic analysis

References

Sfeir A; Kabir S; van Overbeek M; Celli GB; de Lange T. 2010. Loss of Rap1 induces telomere recombination in the absence of NHEJ or a DNA damage signal. Science 327(5973):1657-61. PubMed: 20339076 MGI: J:158884

Additional - Terf2ip^tm1.1Tdl related

Martinez P; Gomez-Lopez G; Garcia F; Mercken E; Mitchell S; Flores JM; de Cabo R; Blasco MA. 2013. RAP1 protects from obesity through its extratelomeric role regulating gene expression. Cell Rep 3(6):2059-74. PubMed: 23791526 MGI: J:199371
Yeung F; Ramirez CM; Mateos-Gomez PA; Pinzaru A; Ceccarini G; Kabir S; Fernandez-Hernando C; Sfeir A. 2013. Nontelomeric role for Rap1 in regulating metabolism and protecting against obesity. Cell Rep 3(6):1847-56. PubMed: 23791522 MGI: J:199372
Zimmermann M; Kibe T; Kabir S; de Lange T. 2014. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. Genes Dev 28(22):2477-91. PubMed: 25344324 MGI: J:217428

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Terf2ip<tm1.1Tdl>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Terf2iptm1.1Tdl

Allele Symbol: Terf2iptm1.1Tdl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Terf2iptm1.1Tdl/Terf2ip+involves: C57BL/6J

no phenotypic analysis

References

Additional - Terf2iptm1.1Tdl related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Terf2ip^tm1.1Tdl

Allele Symbol: Terf2ip^tm1.1Tdl

Genotype: Terf2ip^tm1.1Tdl/Terf2ip⁺
involves: C57BL/6J

Additional - Terf2ip^tm1.1Tdl related