STOCK Hprt^{tm50(Ple55-lacZ)Ems}/Mmjax

Stock number: 012335
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple55-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human DCX gene direct expression of β-galactosidase.

Allele

Symbol: Hprt^{tm50(Ple55-lacZ)Ems}
Name: targeted mutation 50, Elizabeth M Simpson
MGI accession ID: MGI:4834832
Generation method: Targeted
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.129P2-Hprt^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed throughout the brain.
Molecular note: The Ple55-lacZ transgene (pEMS1526) was designed with the 3877 bp Ple55 minipromoter (DCX-D; derived from a portion of the promoter and an upstream putative regulatory element from the doublecortin (DCX) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS3879 has been established.