These mice have the Ple55-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human DCX gene direct expression of β-galactosidase.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Hprt | hypoxanthine guanine phosphoribosyl transferase |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | lacZ is expressed throughout the brain. |
Allele Name | targeted mutation 50, Elizabeth M Simpson |
---|---|
Allele Type | Targeted |
Allele Synonym(s) | DCX-D-lacZ; Hprttm50(mEMS3879)Ems; Ple55 |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | DCX, doublecortin, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed throughout the brain. |
Strain of Origin | (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS3879 has been established. |
Molecular Note | The Ple55-lacZ transgene (pEMS1526) was designed with the 3877 bp Ple55 minipromoter (DCX-D; derived from a portion of the promoter and an upstream putative regulatory element from the doublecortin (DCX) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with wildtype mice from the colony or with 129S1/SvImJ inbred mice. Of note, the donating investigator reports that backcrossing these mutant mice to C57BL/6J inbred mice for more than three generations resulted in absence of hemizygous males from the (N4) litters.
When using the STOCK Hprttm50(Ple55-lacZ)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32942 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.