NOD/ShiLt-Tg(GFAP-Cd274)5Mdos/J mice, commonly referred to as GFAP-tg, express Cd274 (B7-H1), under the control of glial fibrillary acidic protein promoter (GFAP). Hemizygous and homozygous mice are viable and fertile. Rt-PCR indicates B7-H1 transgenic expression appears in neuronal tissues, including the brain and sciatic nerve, pancreas, colon, salivary gland and purified or cultured peri-islet Schwann cells (pSC). Protein expression does not correlate with transcription profiles as protein expression was only found to be elevated in the pSC and brains of transgenic mice when compared to wildtype mice. At 4-weeks of age, transgenic and non-transgenic mice exhibit no difference in glucose and insulin sensitivity tests. By 8-weeks of age, there was enhanced loss of pSC in transgenic females; which followed elevated GFAP autoreactivity. Insulitis is enhanced and more invasive in the transgenic female when compared to wildtype controls (Males not reported). By 19 weeks of age transgenic female mice exhibit accelerated diabetes onset. Splenocytes of diabetic transgenic mice adoptively transferred into NOD-scid recipients results in earlier diabetes onset than diabetic NOD splenocytes transferred into NOD-scid recipients This transgenic mouse strain may be useful for studying the role of peri-islet Schwann cells as modulators of islet inflammation leading to type 1 diabetes.

These GFAP-tg, coisogenic, NOD mice are hemizygous for a transgene encoding Cd274 cDNA inserted into a GFAP expression cassette. Transgenic mice may be useful for studying the role of peri-islet Schwann cells as modulators of islet inflammation leading to type 1 diabetes.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

<a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> for more information.