These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)) and may be useful in generating conditional mutants of Dicer1.
Gregory Hannon, Cold Spring Harbor Laboratory
These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)).When bred to mice expressing cre recombinase, exons 22 and 23 (encoding the RNase III domain) are deleted in the cre-expressing tissues of the offspring, resulting in a non-functional allele. This strain may be useful in studies of skin and hair follicle biology as well as mammalian organogenesis.
4E4 embryonic stem cells (129S7/SvEvBrd-Hprt
|Allele Name||targeted mutation 1, Sarah E Millar|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||DicerflEx22-23; Dicerflox|
|Gene Symbol and Name||Dicer1, dicer 1, ribonuclease type III|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|General Note||Phenotypic Similarity to Human Syndrome: Glomerulocystic Disease J:229188.|
|Molecular Note||A floxed Dicer1 allele was created in a stem cell line (4E4) which contained a null Dicer1 allele, Dicer1tm1Ghan. A Dicer1 allele with exons 22 and 23 flanked by loxP sites replaced the remaining wild-type Dicer1 allele in the ES cells. The resulting ES cells were used to create conditional mice homozygous for the floxed Dicer1 allele.|
|Mutations Made By|| |
Sarah Millar, University of Pennsylvania School of Med
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129S7-Dicer1tm1Smr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012284 in your Materials and Methods section.