These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)) and may be useful in generating conditional mutants of Dicer1.
Gregory Hannon, Cold Spring Harbor Laboratory
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Dicer1 | dicer 1, ribonuclease type III |
These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)).When bred to mice expressing cre recombinase, exons 22 and 23 (encoding the RNase III domain) are deleted in the cre-expressing tissues of the offspring, resulting in a non-functional allele. This strain may be useful in studies of skin and hair follicle biology as well as mammalian organogenesis.
4E4 embryonic stem cells (129S7/SvEvBrd-Hprt
Allele Name | targeted mutation 1, Sarah E Millar |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | DicerflEx22-23; Dicerflox |
Gene Symbol and Name | Dicer1, dicer 1, ribonuclease type III |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 12 |
General Note | Phenotypic Similarity to Human Syndrome: Glomerulocystic Disease J:229188. |
Molecular Note | A floxed Dicer1 allele was created in a stem cell line (4E4) which contained a null Dicer1 allele, Dicer1tm1Ghan. A Dicer1 allele with exons 22 and 23 flanked by loxP sites replaced the remaining wild-type Dicer1 allele in the ES cells. The resulting ES cells were used to create conditional mice homozygous for the floxed Dicer1 allele. |
Mutations Made By | Sarah Millar, University of Pennsylvania School of Med |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129S7-Dicer1tm1Smr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012284 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dicer1<tm1Smr> |
Frozen Mouse Embryo | B6;129S7-Dicer1<tm1Smr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Dicer1<tm1Smr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S7-Dicer1<tm1Smr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S7-Dicer1<tm1Smr>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.