B6;129S7-Dicer1^tm1Smr/J

Stock number: 012284
Research areas: Research Tools

Description

These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)) and may be useful in generating conditional mutants of Dicer1.

Detailed description

These mice carry a floxed allele of Dicer1 (Dicer1, Dcr-1 homolog (Drosophila)).When bred to mice expressing cre recombinase, exons 22 and 23 (encoding the RNase III domain) are deleted in the cre-expressing tissues of the offspring, resulting in a non-functional allele. This strain may be useful in studies of skin and hair follicle biology as well as mammalian organogenesis.

Development

4E4 embryonic stem cells (129S7/SvEvBrd-Hprt b-m2 background derived from AB2.2) heterozygously carrying a PGK-neomycin cassette in place of exon 23 (previously called exon 21) were electroporated with a conditional targeting construct composed of an Frt-flanked PGK-puromycin cassette followed by floxed exons 22 and 23 (encoding the RNaseIII domain). Targeted ES cells were microinjected into C57BL/6 blastocysts. Resultant animals carrying only the floxed allele were selected. This strain was backcrossed once to C57BL/6 by the donating laboratory.

Allele

Symbol: Dicer1^tm1Smr
Name: targeted mutation 1, Sarah E Millar
MGI accession ID: MGI:3641051
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Dicer^flox; Dicer^flEx22-23
Marker symbol: Dicer1
Marker name: dicer 1, ribonuclease type III
Chromosome: 12
Strain of origin: 129S7/SvEvBrd-Hprt^b-m2
Molecular note: A floxed Dicer1 allele was created in a stem cell line (4E4) which contained a null Dicer1 allele, Dicer1^tm1Ghan. A Dicer1 allele with exons 22 and 23 flanked by loxP sites replaced the remaining wild-type Dicer1 allele in the ES cells. The resulting ES cells were used to create conditional mice homozygous for the floxed Dicer1 allele.
General note: Phenotypic Similarity to Human Syndrome: Glomerulocystic Disease J:229188.