STOCK Tg(Piwil1)1Ghan/J

Stock number: 012278
Research areas: Research Tools

Description

This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. This strain may be helpful in studies of stem cell differentiation and spermatogenesis.

Detailed description

This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately postnatal day 14 (P14) in the pachytene stage of male germ cell meiosis and continues to adulthood. This strain may be helpful in studies of stem cell differentiation and spermatogenesis.

Development

Three FLAG and one hemagglutinin (HA) tag were inserted at the N-terminus of the mouse Piwil1 (Miwi) gene in BAC RP23-339N20. The linearized BAC was injected into B6SJLF1 hybrid oocytes to generate transgenic animals. The strain was maintained on a mixed C57BL/6, SJL and outbred CD1 background by the donating laboratory.

Allele

Symbol: Tg(Piwil1)1Ghan
Name: transgene insertion 1, Gregory J Hannon
MGI accession ID: MGI:4415288
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: 3xFLAG-HA-MIWI; Miwi-FLAG-HA; Tg(Piwil1)RP23-339N20Ghan
Marker symbol: Tg(Piwil1)1Ghan
Marker name: transgene insertion 1, Gregory J Hannon
Marker synonyms: 3xFLAG-HA-MIWI; Miwi-FLAG-HA
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: Piwil1,piwi-like RNA-mediated gene silencing 1,mouse, laboratory; FLAG, FLAG octapeptide,; HA tag,HA tag,
Molecular note: Three FLAG and one hemagglutinin (HA) tag were inserted at the N-terminus of the mouse Piwil1 (Miwi) gene in BAC RP23-339N20. The linearized BAC was injected into B6SJLF1 hybrid oocytes to generate transgenic animals.