This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. This strain may be helpful in studies of stem cell differentiation and spermatogenesis.
Gregory Hannon, Cold Spring Harbor Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This FLAG- and hemagglutinin (HA)-tagged Piwil1 (piwi-like homolog 1 (Drosophila); also known as Miwi) transgenic line enables researchers to selectively purify piwi-interacting RNA (piRNA) complexes using antibody-coated beads specific for the tagged elements. Expression, under the control of the transgene's native promoter, reproduces the developmentally timed pattern and subcellular localization of the endogenous protein. It begins at approximately postnatal day 14 (P14) in the pachytene stage of male germ cell meiosis and continues to adulthood. This strain may be helpful in studies of stem cell differentiation and spermatogenesis.
Three FLAG and one hemagglutinin (HA) tag were inserted at the N-terminus of the mouse Piwil1 (Miwi) gene in BAC RP23-339N20. The linearized BAC was injected into B6SJLF1 hybrid oocytes to generate transgenic animals. The strain was maintained on a mixed C57BL/6, SJL and outbred CD1 background by the donating laboratory.
Expressed Gene | Piwil1, piwi-like RNA-mediated gene silencing 1, mouse, laboratory |
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Expressed Gene | FLAG, FLAG octapeptide, |
Expressed Gene | HA tag, HA tag, |
Site of Expression |
Allele Name | transgene insertion 1, Gregory J Hannon |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 3xFLAG-HA-MIWI; Miwi-FLAG-HA; Tg(Piwil1)RP23-339N20Ghan |
Gene Symbol and Name | Tg(Piwil1)1Ghan, transgene insertion 1, Gregory J Hannon |
Gene Synonym(s) | |
Promoter | Piwil1, piwi-like RNA-mediated gene silencing 1, mouse, laboratory |
Expressed Gene | Piwil1, piwi-like RNA-mediated gene silencing 1, mouse, laboratory |
Expressed Gene | FLAG, FLAG octapeptide, |
Expressed Gene | HA tag, HA tag, |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | Three FLAG and one hemagglutinin (HA) tag were inserted at the N-terminus of the mouse Piwil1 (Miwi) gene in BAC RP23-339N20. The linearized BAC was injected into B6SJLF1 hybrid oocytes to generate transgenic animals. |
When maintained as a live colony, hemizygotes or homozygotes may be bred.
When using the STOCK Tg(Piwil1)1Ghan/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012278 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Piwil1)1Ghan |
Frozen Mouse Embryo | STOCK Tg(Piwil1)1Ghan/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Piwil1)1Ghan/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Piwil1)1Ghan/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Piwil1)1Ghan/J Frozen Embryo | $3373.50 |
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