B6.Cg-Tg(Cdh16-cre)91Igr/J

Stock number: 012237
Product name: Ksp1.3/Cre
Strain synonyms: Ksp-Cre
Research areas: Research Tools

Description

These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp1.3) promoter. Cre recombinase expression is detected in epithelial cells of the developing kidney and genitourinary tract and in the renal tubules of adult mice. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies.

Detailed description

Hemizygous and homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter. Cre recombinase expression follows expression of the endogenous gene and is detected in the epithelial cells of developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Mullerian duct. In the adult mouse expression is limited to the renal tubules especially the collecting ducts, loops of Henle and distal tubules. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in kidney-specific gene targeting and cell lineage studies.

Development

A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse cadherin 16 (Cdh16 or Ksp-cadherin) promoter was introduced into ICR fertilized mouse eggs. Founder line 91 was subsequently established and crossed to a C57BL/6 (see SNP note below) for more than 20 generations by the donating laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Allele

Symbol: Tg(Cdh16-cre)91Igr
Name: transgene insertion 91, Peter Igarashi
MGI accession ID: MGI:2665300
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Ksp Cre; Ksp1.3/Cre; Ksp1.3-Cre; KspCre; Ksp-Cre
Marker symbol: Tg(Cdh16-cre)91Igr
Marker name: transgene insertion 91, Peter Igarashi
Marker synonyms: Ksp1.3/Cre; Ksp1.3-Cre; KspCre; Ksp-Cre
Chromosome: UN
Strain of origin: ICR
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cdh16 promoter drives transgene expression in the developing kidney and genitourinary tract.
Molecular note: The transgene consists of 1341 bp of the Cdh16 5' flanking region, a beta-globin minimal promoter, and a cre recombinase sequence. The Cdh16 promoter was used to drive transgene expression in the developing kidney and genitourinary tract.
General note: This transgenic line is also representative of the cre expression pattern in line 38.