These mice carry a floxed allele of Cdc25b (cell division cycle 25 homolog B (S. pombe)). When bred to mice with a cre recombinase gene, exons 2-14 of the targeted gene are deleted in the cre expressing tissues of the offspring. This strain may be useful in studies of cell division and oocyte maturation.
Exons 2-14 of the targeted gene were flanked by loxP sites in 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 once by the donating laboratory.
|Allele Name||targeted mutation 1, Helen Piwnica-Worms|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cdc25b, cell division cycle 25B|
|Strain of Origin||129X1/SvJ|
|Molecular Note||Exons 2-14 of the targeted gene were flanked by loxP sites. Cre-mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Dr. Helen Piwnica-Worms, MD Anderson Cancer Center
When maintained as a live colony, heterozygotes may be bred.
When using the B6;129X1-Cdc25btm1Hpw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011129 in your Materials and Methods section.