B6.Cg-Tg(Atoh1-cre)1Bfri/J

Stock number: 011104
Product name: Math1-Cre
Research areas: Neurobiology Research, Research Tools

Description

These transgenic mice have the mouse atonal homolog 1 (Drosophila) (Atoh1-cre or Math1-cre) regulatory sequences directing Cre recombinase expression primarily in precursors of granule cell neurons of the cerebellum and dorsal hindbrain/spinal cord in the dp1 domain

Detailed description

Math1-Cre (Atoh1-Cre) transgenic mice have the mouse Atoh1 (atonal homolog 1 (Drosophila)) regulatory sequences directing Cre recombinase expression primarily in precursors of granule cell neurons of the cerebellum and dorsal hindbrain/spinal cord in the dp1 domain. When bred with mice containing sequences flanked by similarly oriented loxP sites, flanked sequences will be deleted in the Cre-expressing tissues of the offspring.

Development

A 1.5 kbp Atoh1 enhancer fragment drives cre expression in this transgenic line. The transgenic vector was introduced to B6CBAF1 embryos. Resultant mice were crossed with outbred Swiss Webster mice, then backcrossed to C57BL/6 for at least eight generations by the donating laboratory.

Allele

Symbol: Tg(Atoh1-cre)1Bfri
Name: transgene insertion 1, Bernd Fritzsch
MGI accession ID: MGI:3775845
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Atoh1-cre)1Bfri
Marker name: transgene insertion 1, Bernd Fritzsch
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: When crossed to a lacZ reporter such as Stock No. 003474, Atoh1-Cre mediated beta-galactosidase is expressed in delaminating sensory neuron precursors as well as undifferentiated epithelial cells at E11 and E12.5. PCR analysis shows expression of Atoh1 in the otocyst as early as E10.5. Analysis of Atoh1-Cre expression in E18.5 embryos and P31 mice reveal beta-galactosidase stain in all hair cells but also in vestibular and cochlear sensory neurons and some supporting cells. A similar expression of Atoh1-LacZ exists in postnatal and adult vestibular and cochlear sensory neurons, and Atoh1 expression in vestibular sensory neurons is confirmed with RT-PCR.
Molecular note: A 1.5 kb Atoh1 enhancer fragment drives cre expression in neurosensory precursors. The transgenic vector was introduced to B6CBAF1 embryos.