These transgenic mice have the mouse atonal homolog 1 (Drosophila) (Atoh1-cre or Math1-cre) regulatory sequences directing Cre recombinase expression primarily in precursors of granule cell neurons of the cerebellum and dorsal hindbrain/spinal cord in the dp1 domain
David H. Rowitch, University of California, San Francisco
Genetic Background | Generation |
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N10+F6
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$278.00 Domestic price for female |
356.51 Domestic price for breeder pair |
Math1-Cre (Atoh1-Cre) transgenic mice have the mouse Atoh1 (atonal homolog 1 (Drosophila)) regulatory sequences directing Cre recombinase expression primarily in precursors of granule cell neurons of the cerebellum and dorsal hindbrain/spinal cord in the dp1 domain. When bred with mice containing sequences flanked by similarly oriented loxP sites, flanked sequences will be deleted in the Cre-expressing tissues of the offspring.
A 1.5 kbp Atoh1 enhancer fragment drives cre expression in this transgenic line. The transgenic vector was introduced to B6CBAF1 embryos. Resultant mice were crossed with outbred Swiss Webster mice, then backcrossed to C57BL/6 for at least eight generations by the donating laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | When crossed to a lacZ reporter such as Stock No. 003474, Atoh1-Cre mediated beta-galactosidase is expressed in delaminating sensory neuron precursors as well as undifferentiated epithelial cells at E11 and E12.5. PCR analysis shows expression of Atoh1 in the otocyst as early as E10.5. Analysis of Atoh1-Cre expression in E18.5 embryos and P31 mice reveal beta-galactosidase stain in all hair cells but also in vestibular and cochlear sensory neurons and some supporting cells. A similar expression of Atoh1-LacZ exists in postnatal and adult vestibular and cochlear sensory neurons, and Atoh1 expression in vestibular sensory neurons is confirmed with RT-PCR. |
Allele Name | transgene insertion 1, Bernd Fritzsch |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Math1::cre; Math1-cre; tgAtoh1creFri |
Gene Symbol and Name | Tg(Atoh1-cre)1Bfri, transgene insertion 1, Bernd Fritzsch |
Gene Synonym(s) | |
Promoter | Atoh1, atonal bHLH transcription factor 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | When crossed to a lacZ reporter such as Stock No. 003474, Atoh1-Cre mediated beta-galactosidase is expressed in delaminating sensory neuron precursors as well as undifferentiated epithelial cells at E11 and E12.5. PCR analysis shows expression of Atoh1 in the otocyst as early as E10.5. Analysis of Atoh1-Cre expression in E18.5 embryos and P31 mice reveal beta-galactosidase stain in all hair cells but also in vestibular and cochlear sensory neurons and some supporting cells. A similar expression of Atoh1-LacZ exists in postnatal and adult vestibular and cochlear sensory neurons, and Atoh1 expression in vestibular sensory neurons is confirmed with RT-PCR. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | A 1.5 kb Atoh1 enhancer fragment drives cre expression in neurosensory precursors. The transgenic vector was introduced to B6CBAF1 embryos. |
Mutations Made By | Bernd Fritzsch, University of Iowa |
When maintained as a live colony, hemizygotes or homozygotes may be bred.
When using the Math1-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #011104 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Atoh1-cre)1Bfri |
Frozen Mouse Embryo | B6.Cg-Tg(Atoh1-cre)1Bfri/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Atoh1-cre)1Bfri/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Atoh1-cre)1Bfri/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Atoh1-cre)1Bfri/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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