These mice possess loxP sites flanking portions of exon 2 and intron 2 of the Ednrb (endothelin receptor type B) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful for studying the variety of functions associated with this gene ranging from coat color to cell signaling, digestive and immune phenotypes.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ednrb | endothelin receptor type B |
These mice possess loxP sites flanking portions of exon 2 and intron 2 of the Ednrb (endothelin receptor type B) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful for studying the variety of functions associated with this gene ranging from coat color to cell signaling, digestive and immune phenotypes.
When crossed with a Sox2-cre transgenic strain, mice homozygous for the resulting allele lack Ednrb expression and have a phenotype identical to mice with the spotted lethal (s-l, piebald lethal) allele.
A LoxP site was inserted upstream of the start codon within the second exon of the targeted gene. A second LoxP site was placed downstream of the second exon, within the second intron. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neo selectable marker. This mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Allele Name | targeted mutation 1.1, Jeremy Nathans |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Ednrb, endothelin receptor type B |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 14 |
Molecular Note | A LoxP site was inserted upstream of the start codon within the second exon of the targeted gene. A second LoxP site was placed downstream of the second exon, within the second intron. Adjacent to the second LoxP site is an Frt site left over after the excision of the PGK-neo selectable marker. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Ednrbtm1.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011080 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ednrb<tm1.1Nat> |
Frozen Mouse Embryo | B6;129-Ednrb<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ednrb<tm1.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ednrb<tm1.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Ednrb<tm1.1Nat>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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