This strain combines a conditional knockout and a alkaline phosphatase (AP) reporter of the frizzled 4 (Fzd4; Fz4) gene. Following cre-mediated recombination, the coding region is excised, and the downstream AP reporter is expressed under the control of the Fzd4 promoter in a cell/tissue-specific manner.
Jeremy Nathans, Johns Hopkins University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Fzd4 | frizzled class receptor 4 |
This strain combines a conditional knockout and an alkaline phosphatase (AP) reporter of the frizzled 4 (Fzd4; Fz4) gene. LoxP sites flank the 5' and 3' UTR's. Without recombination, homozygotes of this allele are indistinguishable from wild type mice and the AP reporter is not expressed. Following cre-mediated recombination, the coding region is excised, and the downstream AP reporter is expressed under the control of the Fzd4 promoter in a cell/tissue-specific manner. Loss of Fzd4 signaling causes defective vascular growth which leads to chronic but reversible silencing of retinal neurons. Loss of expression in all endothelial cells disrupts the blood brain barrier in the cerebellum. This strain may be useful in studies of vascular growth, remodeling, maintenance and disease.
LoxP sites are inserted before the start codon, and after the 3'UTR. The coding sequence of human placenta alkaline phosphatase gene and rabbit beta-globin 3' UTR were inserted downstream the 3' LoxP site. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells and the strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Allele Name | targeted mutation 2.1, Jeremy Nathans |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Fz4CKOAP |
Gene Symbol and Name | Fzd4, frizzled class receptor 4 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | One loxP site was inserted in the 5' UTR and another was inserted 3' of the 3' UTR. An alkaline phosphatase sequence was inserted 3' of the last loxP site and was followed by an frt flanked PGK-neo cassette. Flp recombinase expression resulted in the excision of the PGK-neo cassette. |
Mutations Made By | Jeremy Nathans, Johns Hopkins University |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6;129-Fzd4tm2.1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011078 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fzd4<tm2Nat> |
Frozen Mouse Embryo | B6;129-Fzd4<tm2.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Fzd4<tm2.1Nat>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Fzd4<tm2.1Nat>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Fzd4<tm2.1Nat>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.