NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CSF2)2Ygy Tg(IL3)1Ygy Tg(KITLG)3Ygy/YgyJGckRolyJ

Stock number: 011066
Product name: NSG-porcine cyokine
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These NSG-porcine cyokine mouse mutant mice may be useful for long-term porcine tissue and stem cell engraftment and transplantation research.

Detailed description

These mutant mice carry the Prkdc^scid and Il2rg^tm1Wjl alleles and the Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenes. The Tg(CSF2)2Ygy transgene contains the porcine colony stimulating factor 2 (granulocyte-macrophage) sequence (CSF2) under the control of the human cytomegalovirus promoter. The Tg(IL3)1Ygy transgene contains the porcine interleukin 3 sequence under the control of the human cytomegalovirus promoter. The Tg(KITLG)3Ygy transgene contains the porcine kit ligand (KITL) under the control of the human cytomegalovirus promoter. The 3 transgenes were coinjected together in the original transgenic strain used to generate this mutant strain.

Development

This strain was generated by crossing 004644 mice with 005557 mice. The resulting mice were intercrossed and maintained as homozygous for the spontaneous allele and homozygous (or hemizygous, as the allele is X-linked) for the targeted mutation allele and hemizygous for the transgenes.
STOCK no. 004644 was developed with the 3 transgenic constructs, Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy/YgyJ, which were coinjected into NIH/Swiss oocytes. Transgenic founders carrying all 3 transgenes were crossed to FVB and then crossed to NOD.CB17-Prkdc^scid for 7 generations prior to intercrossing.
For STOCK no. 005557, female NOD.CB17-Prkdc^scid/J (Stock No. 001303) mice were crossed with male B6.129S4-Il2rg^tm1Wjl/J mice (Stock No. 003174). The resulting male mice heterozygous for the Prkdcscid allele and hemizygous for the Il2rg^tm1Wjl allele were crossed to female NOD.CB17-Prkdc^scid/J mice for eight generations before being made homozygous (or hemizygous) for both alleles.

Allele

Symbol: Tg(KITLG)3Ygy
Name: transgene insertion 3, Yong-guang Yang
MGI accession ID: MGI:3057265
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(KITLG)3Ygy
Marker name: transgene insertion 3, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: KITLG,KIT ligand,human
Molecular note: The transgene construct contains porcine kit ligand (KITL) controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(IL3)1Ygy and Tg(CSF2)2Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Tg(CSF2)2Ygy
Name: transgene insertion 2, Yong-guang Yang
MGI accession ID: MGI:3057263
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(CSF2)2Ygy
Marker name: transgene insertion 2, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: CSF2,colony stimulating factor 2 (granulocyte-macrophage),pig, domestic
Molecular note: The transgene construct contains the porcine colony stimulating factor 2 (granulocyte-macrophage) sequence (CSF2) controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(IL3)1Ygy and Tg(KITLG)3Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Tg(IL3)1Ygy
Name: transgene insertion 1, Yong-guang Yang
MGI accession ID: MGI:3057194
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(IL3)1Ygy
Marker name: transgene insertion 1, Yong-guang Yang
Chromosome: UN
Strain of origin: NIH Swiss
Expressed genes: IL3,interleukin 3 (colony-stimulating factor, multiple),pig, domestic
Molecular note: The transgene construct contains the porcine interleukin 3 sequence controlled by the human cytomegalovirus promoter. The Tg(IL3)1Ygy, Tg(CSF2)2Ygy and Tg(KITLG)3Ygy transgenic constructs were co-injected into NIH/Swiss oocytes in David Bodine's lab at NCI.
General note: White blood cells from mice that carry this transgene in conjunction with Tg(CSF2)2Ygy and Tg(KITLG)3Ygy and that are also homozygous for Prkdc^scid are CD4^-, CD8^-, CD3^-, IgM^-, CD19^- and Mac-1⁺ by flow cytometric (FMC) analysis.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Synonyms: scid
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Marker synonyms: DNAPDcs; DNAPK; DNA-PK; DNAPKc; DNA-PKC; DNA-PKcs; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; slip; XRCC7
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Il2rg^tm1Wjl
Name: targeted mutation 1, Warren J Leonard
MGI accession ID: MGI:1857455
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: [KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Marker symbol: Il2rg
Marker name: interleukin 2 receptor, gamma chain
Marker synonyms: [g]c; Ab2-183; Cd132; CIDX; common cytokine receptor gamma chain; common gamma chain; gamma C receptor; gamma(c); gammaC; gc; IL-2RG; IMD4; P64; SCIDX; SCIDX1
Chromosome: X
Strain of origin: 129S4/SvJae
Site of expression: Primarily lymphoid cells.
Molecular note: A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.