Mice that are homozygous for this Ctbp1 (C-terminal binding protein 1) targeted mutation are smaller than wildtype littermates. Approximately 23% of homozygous mice die by 20 days of age. This mutant mouse strain may be useful in studies of development.
Jeffrey D. Hildebrand, University of Pittsburgh
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are 30% smaller than wildtype littermates and approximately 23% of homozygous mice die by 20 days of age. Although surviving homozygotes are fertile, they do not breed well. No gene product (protein) is detected by Western blot analysis of homozygous embryos.
A targeting vector containing a floxed PGKneo selection cassette was used to disrupt the exon encoding amino acids 55 to 102. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The strain was maintained as a heterozygote. Upon arrival at The Jackson Laboratory, the mice were crossed once to C57BL/6J to establish the colony.
|Allele Name||targeted mutation 1, Philippe Soriano|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ctbp1, C-terminal binding protein 1|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||The exon encoding amino acids 55 through 102 was replaced with a floxed neomycin selection cassette. RT-PCR and sequence analyses showed that the disrupted exon was excised and the resultant splicing of the adjacent exons introduced a frameshift mutation. The absence of protein was verifed by Western blot analysis of lysates obtained from homozygous mutant embyros.|
|Mutations Made By|| |
Jeffrey Hildebrand, University of Pittsburgh
When maintaining a live colony, these mice can be bred as heterozygotes. Approximately 23% of homozygous mice die by 20 days of age. Although surviving homozygotes are fertile, they do not breed well.
When using the B6;129S4-Ctbp1tm1Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011054 in your Materials and Methods section.