Mice that are homozygous for this Ctbp2 (C-terminal binding protein 2) gene trap mutation exhibit an embryonic lethal phenotype, extraembryonic vasculature defects, small size, axial truncations, delayed forebrain and midbrain development, dilated pericardium and abnormal heart development. This mutant mouse strain may be useful in studies of embryonic heart and brain development.
Jeffrey D. Hildebrand, University of Pittsburgh
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Reporter, Null/Knockout) | Ctbp2 | C-terminal binding protein 2 |
Mice that are heterozygous for the gene trap mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have an embryonic lethal phenotype, failing to survive past embryonic day 10.5.
No gene product (protein) is detected by Western blot analysis of homozygous embryos.
Homozygous embryos exhibit extraembryonic vasculature defects, small size, axial truncations, delayed forebrain and midbrain development, dilated pericardium and abnormal heart development. Beta-galactosidase expression and staining mimics the expected endogenous gene expression pattern.
A gene trap construct containing neomycin resistance and beta-galactosidase (lacZ) genes was introduced by retroviral infection into 129S4/SvJaeSor derived embryonic stem (ES) cells. The construct integrated downstream of the putative start codon. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The strain was maintained as a heterozygote. Upon arrival at The Jackson Laboratory, the mice were crossed once to C57BL/6J to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | LacZ expression and staining mimics the endogenous targeted gene pattern. |
Allele Name | gene trap ROSA61, Philippe Soriano |
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Allele Type | Gene trapped (Reporter, Null/Knockout) |
Allele Synonym(s) | Ctbp2-; Ctbp2Gt(ROSA)61Sor; Ctbp2Gt(ROSABetageo)61Sor |
Gene Symbol and Name | Ctbp2, C-terminal binding protein 2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LacZ expression and staining mimics the endogenous targeted gene pattern. |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 7 |
Molecular Note | A retroviral gene trap vector containing a lacZ/neo fusion gene integrated downstream of an exon that is common to both isoforms. The absence of protein was verifed by Western blot analysis of lysates obtained from homozygous mutant embyros. |
Mutations Made By | Jeffrey Hildebrand, University of Pittsburgh |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype, failing to survive past embryonic day 10.5.
When using the B6;129S4-Ctbp2Gt(ROSA61)Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011052 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild type for Ctbp2<Gt(ROSA)61Sor> |
Frozen Mouse Embryo | B6;129S4-Ctbp2<Gt(ROSA61)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Ctbp2<Gt(ROSA61)Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Ctbp2<Gt(ROSA61)Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Ctbp2<Gt(ROSA61)Sor>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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