B6;129S4-Ctbp2^{Gt(ROSA61)Sor}/J

Stock number: 011052
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

Mice that are homozygous for this Ctbp2 (C-terminal binding protein 2) gene trap mutation exhibit an embryonic lethal phenotype, extraembryonic vasculature defects, small size, axial truncations, delayed forebrain and midbrain development, dilated pericardium and abnormal heart development. This mutant mouse strain may be useful in studies of embryonic heart and brain development.

Detailed description

Mice that are heterozygous for the gene trap mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have an embryonic lethal phenotype, failing to survive past embryonic day 10.5. No gene product (protein) is detected by Western blot analysis of homozygous embryos. Homozygous embryos exhibit extraembryonic vasculature defects, small size, axial truncations, delayed forebrain and midbrain development, dilated pericardium and abnormal heart development. Beta-galactosidase expression and staining mimics the expected endogenous gene expression pattern.

Development

A gene trap construct containing neomycin resistance and beta-galactosidase (lacZ) genes was introduced by retroviral infection into 129S4/SvJaeSor derived embryonic stem (ES) cells. The construct integrated downstream of the putative start codon. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The strain was maintained as a heterozygote. Upon arrival at The Jackson Laboratory, the mice were crossed once to C57BL/6J to establish the colony.

Allele

Symbol: Ctbp2^{Gt(ROSA61)Sor}
Name: gene trap ROSA61, Philippe Soriano
MGI accession ID: MGI:2183646
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Marker symbol: Ctbp2
Marker name: C-terminal binding protein 2
Chromosome: 7
Strain of origin: 129S4/SvJaeSor
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: LacZ expression and staining mimics the endogenous targeted gene pattern.
Molecular note: A retroviral gene trap vector containing a lacZ/neo fusion gene integrated downstream of an exon that is common to both isoforms. The absence of protein was verifed by Western blot analysis of lysates obtained from homozygous mutant embyros.