B6.FVB-Tg(Myh6-cre)2182Mds/J

Stock number: 011038
Product name: αMyHC-Cre
Strain synonyms: MCH-cre
Research areas: Cardiovascular Research, Research Tools

Description

The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of cre in this transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells.

Detailed description

The cardiac-specific murine alpha myosin-heavy chain (Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of cre in this transgenic strain. Breeding this mouse with another carrying loxP-flanked sequences results in the deletion of the flanked sequences in the offspring. The promoter induces greater than 90% recombination in cardiac muscle cells. No recombination is observed in liver, lung, skeletal muscle (quadriceps), and spleen, or in extraneous cell types in the heart.

Development

Cardiac-specific mouse alpha myosin heavy chain promoter drives expression of cre. This strain was created an FVB background then backcrossed to C57BL/6 for ten generations by the donating laboratory.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (4 of 4 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.

Allele

Symbol: Tg(Myh6-cre)2182Mds
Name: transgene insertion 2182, Michael D Schneider
MGI accession ID: MGI:2386742
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Myh6-cre)2182Mds
Marker name: transgene insertion 2182, Michael D Schneider
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: cre recombinase is expressed in cardiac tissue.
Molecular note: The transgenic construct contained the Myh7 3' untranslated region, the Myhca promoter, Myhca noncoding exons 1 and 2, and the exon 3 splice acceptor site 180 bp upstream and driving the expression of the cre recombinase sequence. The Myhca promoter drives expression in cardiac tissue.