The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of cre in this transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells.
Michael Schneider, Imperial College London
Genetic Background | Generation |
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N11+N5F3
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
The cardiac-specific murine alpha myosin-heavy chain (Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter drives expression of cre in this transgenic strain. Breeding this mouse with another carrying loxP-flanked sequences results in the deletion of the flanked sequences in the offspring. The promoter induces greater than 90% recombination in cardiac muscle cells. No recombination is observed in liver, lung, skeletal muscle (quadriceps), and spleen, or in extraneous cell types in the heart.
Cardiac-specific mouse alpha myosin heavy chain promoter drives expression of cre. This strain was created an FVB background then backcrossed to C57BL/6 for ten generations by the donating laboratory.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (4 of 4 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | cre recombinase is expressed in cardiac tissue. |
Allele Name | transgene insertion 2182, Michael D Schneider |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | alphaMhc-Cre; alpha-MHC-Cre+; alphaMyHC-Cre; MCH-cre; MHCalphaCre; MHCcre; MHC-Cre; Tg(Myhca-cre)2182Mds |
Gene Symbol and Name | Tg(Myh6-cre)2182Mds, transgene insertion 2182, Michael D Schneider |
Gene Synonym(s) | |
Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | cre recombinase is expressed in cardiac tissue. |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The transgenic construct contained the Myh7 3' untranslated region, the Myhca promoter, Myhca noncoding exons 1 and 2, and the exon 3 splice acceptor site 180 bp upstream and driving the expression of the cre recombinase sequence. The Myhca promoter drives expression in cardiac tissue. |
Mutations Made By | Michael Schneider, Imperial College London |
Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (March, 2016).
When using the αMyHC-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #011038 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Myh6-cre)2182Mds |
Frozen Mouse Embryo | B6.FVB-Tg(Myh6-cre)2182Mds/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Myh6-cre)2182Mds/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Myh6-cre)2182Mds/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Myh6-cre)2182Mds/J Frozen Embryos | $3373.50 |
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