These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene as well as expression of the BimEL isoform. Other isoforms, including BimL and BimS, are expressed. These mice represent a model for the analysis of BimEL loss-of-function.
Roger J Davis, University of Massachusetts Medical Sch.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Applicable) | Bcl2l11 | BCL2-like 11 (apoptosis facilitator) |
These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene. Alternative splicing to include exon 3 is required for expression of the BimEL isoform, lacking in these mice. Other isoforms, including BimL and BimS, are expressed however. These mice represent a model for the analysis of BimEL loss-of-function.
Exon 3 was deleted and a loxP-flanked neomycin resistance cassette was introduced to intron 4. TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed ten times to C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 4, Roger J Davis |
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Allele Type | Targeted (Not Applicable) |
Allele Synonym(s) | BimdeltaEL; BimL-only |
Gene Symbol and Name | Bcl2l11, BCL2-like 11 (apoptosis facilitator) |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A floxed neo cassette was inserted within intron 4, and genomic sequence with deleted alternatively spliced exon 3 was introduced by homologous recombination. Replaced mutation caused the entire exon 3, necessary for BimEL isoform transcription, to be deleted. The neo cassette was then excised with cre recombinase, leaving a single loxP site in intron 4. By immunoblot analyses, no BimEL proteins were expressed; however, increased amounts of BimL proteins was expressed. This is BimEL isoform specific knock-out allele. |
Mutations Made By | Roger Davis, University of Massachusetts Medical Sch. |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S6-Bcl2l11tm4Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011027 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bcl2l11<tm4Rjd> |
Frozen Mouse Embryo | B6.129S6-Bcl2l11<tm4Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bcl2l11<tm4Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bcl2l11<tm4Rjd>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Bcl2l11<tm4Rjd>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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