B6.129S6-Bcl2l11^tm4Rjd/J

Stock number: 011027
Research areas: Apoptosis Research

Description

These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene as well as expression of the BimEL isoform. Other isoforms, including BimL and BimS, are expressed. These mice represent a model for the analysis of BimEL loss-of-function.

Detailed description

These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene. Alternative splicing to include exon 3 is required for expression of the BimEL isoform, lacking in these mice. Other isoforms, including BimL and BimS, are expressed however. These mice represent a model for the analysis of BimEL loss-of-function.

Development

Exon 3 was deleted and a loxP-flanked neomycin resistance cassette was introduced to intron 4. TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed ten times to C57BL/6 by the donating laboratory.

Allele

Symbol: Bcl2l11^tm4Rjd
Name: targeted mutation 4, Roger J Davis
MGI accession ID: MGI:3800472
Generation method: Targeted
Attributes: Not Applicable
Marker symbol: Bcl2l11
Marker name: BCL2-like 11 (apoptosis facilitator)
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed neo cassette was inserted within intron 4, and genomic sequence with deleted alternatively spliced exon 3 was introduced by homologous recombination. Replaced mutation caused the entire exon 3, necessary for BimEL isoform transcription, to be deleted. The neo cassette was then excised with cre recombinase, leaving a single loxP site in intron 4. By immunoblot analyses, no BimEL proteins were expressed; however, increased amounts of BimL proteins was expressed. This is BimEL isoform specific knock-out allele.