These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene as well as expression of the BimEL isoform. Other isoforms, including BimL and BimS, are expressed. These mice represent a model for the analysis of BimEL loss-of-function.
Roger J Davis, University of Massachusetts Medical Sch.
These targeted mutation mice lack exon 3 of the Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) gene. Alternative splicing to include exon 3 is required for expression of the BimEL isoform, lacking in these mice. Other isoforms, including BimL and BimS, are expressed however. These mice represent a model for the analysis of BimEL loss-of-function.
Exon 3 was deleted and a loxP-flanked neomycin resistance cassette was introduced to intron 4. TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed ten times to C57BL/6 by the donating laboratory.
|Allele Name||targeted mutation 4, Roger J Davis|
|Allele Type||Targeted (Not Applicable)|
|Allele Synonym(s)||BimdeltaEL; BimL-only|
|Gene Symbol and Name||Bcl2l11, BCL2-like 11 (apoptosis facilitator)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A floxed neo cassette was inserted within intron 4, and genomic sequence with deleted alternatively spliced exon 3 was introduced by homologous recombination. Replaced mutation caused the entire exon 3, necessary for BimEL isoform transcription, to be deleted. The neo cassette was then excised with cre recombinase, leaving a single loxP site in intron 4. By immunoblot analyses, no BimEL proteins were expressed; however, increased amounts of BimL proteins was expressed. This is BimEL isoform specific knock-out allele.|
|Mutations Made By|| |
Roger Davis, University of Massachusetts Medical Sch.
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S6-Bcl2l11tm4Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011027 in your Materials and Methods section.