These mice express a mutated Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) protein in which JNK phosphorylation site Thr112 is replaced with Ala. This mutation suppresses JNK-mediated Bim-induced apoptosis.
Roger J Davis, University of Massachusetts Medical Sch.
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Bcl2l11 | BCL2-like 11 (apoptosis facilitator) |
These mice express a mutated Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) protein in which JNK phosphorylation site Thr112 is replaced with Ala. This mutation suppresses JNK-mediated Bim-induced apoptosis. This mutation does not affect expression of Bim in the spleen.
A loxP-flanked neomycin resistance cassette was placed in intron 4 and threonine 112 was replaced with alanine in exon 4 (AACCCC to GGCGCC), creating a novel KasI restriction site. TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Allele Name | targeted mutation 3, Roger J Davis |
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Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | BimT112A; BimTA |
Gene Symbol and Name | Bcl2l11, BCL2-like 11 (apoptosis facilitator) |
Gene Synonym(s) | |
Promoter | Bcl2l11, BCL2-like 11 (apoptosis facilitator), mouse, laboratory |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A floxed neo cassette was inserted within intron 4, and a specific mutation in exon 4 was introduced by homologous recombination. Mutation resulted in one site of JNK phosphorylation (Thr-112) in exon 4 to be substituted with an Ala (T112A). The neo cassette was then excised with cre recombines leaving a single loxP site in intron 4. Expression of normal amounts of mutant proteins was confirmed by immunoblot. |
Mutations Made By | Roger Davis, University of Massachusetts Medical Sch. |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S-Bcl2l11tm3Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011026 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bcl2l11<tm3Rjd> |
Frozen Mouse Embryo | B6.129S-Bcl2l11<tm3Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S-Bcl2l11<tm3Rjd>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S-Bcl2l11<tm3Rjd>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S-Bcl2l11<tm3Rjd>/J | $3373.50 |
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