B6.129S-Bcl2l11^tm3Rjd/J

Stock number: 011026
Research areas: Apoptosis Research

Description

These mice express a mutated Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) protein in which JNK phosphorylation site Thr112 is replaced with Ala. This mutation suppresses JNK-mediated Bim-induced apoptosis.

Detailed description

These mice express a mutated Bim (Bcl2l11, BCL2-like 11 (apoptosis facilitator)) protein in which JNK phosphorylation site Thr112 is replaced with Ala. This mutation suppresses JNK-mediated Bim-induced apoptosis. This mutation does not affect expression of Bim in the spleen.

Development

A loxP-flanked neomycin resistance cassette was placed in intron 4 and threonine 112 was replaced with alanine in exon 4 (AACCCC to GGCGCC), creating a novel KasI restriction site. TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.

Allele

Symbol: Bcl2l11^tm3Rjd
Name: targeted mutation 3, Roger J Davis
MGI accession ID: MGI:3800462
Generation method: Targeted
Attributes: Not Specified
Synonyms: Bim^TA; Bim^T112A
Marker symbol: Bcl2l11
Marker name: BCL2-like 11 (apoptosis facilitator)
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed neo cassette was inserted within intron 4, and a specific mutation in exon 4 was introduced by homologous recombination. Mutation resulted in one site of JNK phosphorylation (Thr-112) in exon 4 to be substituted with an Ala (T112A). The neo cassette was then excised with cre recombines leaving a single loxP site in intron 4. Expression of normal amounts of mutant proteins was confirmed by immunoblot.