These mice express Bmf (BCL2 modifying factor) with a targeted gain-of-function Ser74Asp mutation in the JNK (Mapk8) phosphorylation site. These mice may be useful in studies of JNK (Mapk8)-induced apoptosis.
Roger J Davis, University of Massachusetts Medical Sch.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Bmf | BCL2 modifying factor |
These mice express Bmf (BCL2 modifying factor) with a targeted gain-of-function Ser74Asp mutation in the JNK (Mapk8) phosphorylation site. This mutation does not affect expression in the spleen.
A loxP-flanked neomycin resistance cassette was introduced to intron 3 and a Ser74Asp (S74D) mutation with novel BamHI restriction site was created in codon 74/exon 3 (CTCAGTCCA to CTGGATCCA). TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Allele Name | targeted mutation 3.1, Roger J Davis |
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Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | BmfS74A; BmfSA |
Gene Symbol and Name | Bmf, BCL2 modifying factor |
Gene Synonym(s) | |
Promoter | Bmf, BCL2 modifying factor, mouse, laboratory |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A floxed neo cassette was inserted within intron 3, and specific nucleotide mutations in exon 3 were introduced by homologous recombination. Mutation resulted in the JNK protein kinase phosphorylation site (Ser-74) in exon 3 to be substituted with an Ala (S74A). The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3. |
Mutations Made By | Roger Davis, University of Massachusetts Medical Sch. |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S6-Bmftm3.1Rjd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #011023 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Bmf<tm3.1Rjd> |
Frozen Mouse Embryo | B6.129S6-Bmf<tm3.1Rjd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm3.1Rjd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm3.1Rjd>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Bmf<tm3.1Rjd>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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