B6.129S6-Bmf^tm3.1Rjd/J

Stock number: 011023
Research areas: Apoptosis Research

Description

These mice express Bmf (BCL2 modifying factor) with a targeted gain-of-function Ser74Asp mutation in the JNK (Mapk8) phosphorylation site. These mice may be useful in studies of JNK (Mapk8)-induced apoptosis.

Detailed description

These mice express Bmf (BCL2 modifying factor) with a targeted gain-of-function Ser74Asp mutation in the JNK (Mapk8) phosphorylation site. This mutation does not affect expression in the spleen.

Development

A loxP-flanked neomycin resistance cassette was introduced to intron 3 and a Ser74Asp (S74D) mutation with novel BamHI restriction site was created in codon 74/exon 3 (CTCAGTCCA to CTGGATCCA). TC1 129S6/SvEvTac-derived embryonic stem (ES) cells were used to create the mutation. The floxed neomycin cassette was excised with germline protamine (Prm) PC3-cre recombinase on a C57BL/6 background, leaving a single loxP site. This strain was backcrossed to C57BL/6 for ten generations by the donating laboratory.

Allele

Symbol: Bmf^tm3.1Rjd
Name: targeted mutation 3.1, Roger J Davis
MGI accession ID: MGI:4421180
Generation method: Targeted
Attributes: Not Specified
Marker symbol: Bmf
Marker name: BCL2 modifying factor
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed neo cassette was inserted within intron 3, and specific nucleotide mutations in exon 3 were introduced by homologous recombination. Mutation resulted in the JNK protein kinase phosphorylation site (Ser-74) in exon 3 to be substituted with an Ala (S74A). The neo cassette was then excised with Cre mediated recombination leaving a single loxP site in intron 3.