JAX Mouse Strain Datasheet - 010984

B6;129P-Upk1b^tm1Pzg/J

Stock No:: 010984

Targeted Mutation

Cryo Recovery

Overview

Also Known As: Upk1b^tm1Zhang

These targeted mutation mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos. This mutant mouse strain may be useful in studies of genitourinary system development.

Donating Investigator

Dr. Pumin Zhang, Baylor College of Medicine

Genetic overview

Genetic Background	Generation

Upk1b^tm1Pzg

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout, Reporter)	Upk1b	uroplakin 1B

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Research Applications

Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

These mutant mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

A targeting vector containing the Red Fluorescent Protein gene and duplicated loxP and FRT site flanked Kanamycin/neomycin selection cassettes was used to disrupt the exon containing the ATG translation start-site. The construct was electroporated into 129P2/OlaHsd derived E14Tg2a.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mutant mice were bred with B6129PF1/J mice (Stock No. 100492) for at least one generation to establish the colony.

Expression Data

Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos.

Control Suggestions

Wild-type from the colony
100492 B6129PF1/J

Additional Information

Considerations for Choosing Controls

Genetics

Upk1b^tm1Pzg

Allele Symbol: Upk1b^tm1Pzg

Allele Name	targeted mutation 1, Pumin Zhang
Allele Type	Targeted (Null/Knockout, Reporter)
Allele Synonym(s)	Upk1b-RFP; Upk1b^tm1Zhang
Gene Symbol and Name	Upk1b, uroplakin 1B
Gene Synonym(s)	AI413235; TSPAN20; Tspan20; UPIB; UPK1; Upk1; Upk1; expressed sequence AI413235; uroplakin
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos.
Strain of Origin	129P2/OlaHsd
Chromosome	16
Molecular Note	A targeting vector containing the Red Fluorescent Protein (RFP) gene followed, in reverse orientation, by two copies of a loxP and frt site-flanked kanamycin and neomycin resistance cassette (loxP-pA-frt-Kan&Neo-frt-loxP) was inserted into the first coding exon of the endogenous gene immediately following the ATG translation initiation codon. Red fluorescent signal is detected in the kidney and gonads of E15.5 heterozygous mutant embryos; fluorescence in the urogenital system mimics the expression pattern of the endogenous gene.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
- Fluorescent Proteins
- Reproductive Biology Research

Mammalian Phenotype Terms by Genotype

Genotype: Upk1b^tm1Pzg/Upk1b⁺
involves: 129P2/OlaHsd * C57BL/6J

normal phenotype

no abnormal phenotype detected
- mice heterozygous for this mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities

References

Additional - Upk1b^tm1Pzg related

Lua I; Li Y; Pappoe LS; Asahina K. 2015. Myofibroblastic Conversion and Regeneration of Mesothelial Cells in Peritoneal and Liver Fibrosis. Am J Pathol 185(12):3258-73. PubMed: 26598235 MGI: J:228136
McMahon A; Zhang P and colleagues, The GenitoUrinary Development Molecular Anatomy Project (GUDMAP). 2010. Summary of mouse strains characterized by GUDMAP consortium Unpublished :. MGI: J:159099

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Upk1b<tm1Zhang>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: Upk1btm1Zhang

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Upk1btm1Pzg

Allele Symbol: Upk1btm1Pzg

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Upk1btm1Pzg/Upk1b+involves: 129P2/OlaHsd * C57BL/6J

normal phenotype

References

Additional - Upk1btm1Pzg related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Upk1b^tm1Zhang

Upk1b^tm1Pzg

Allele Symbol: Upk1b^tm1Pzg

Genotype: Upk1b^tm1Pzg/Upk1b⁺
involves: 129P2/OlaHsd * C57BL/6J

Additional - Upk1b^tm1Pzg related