B6;129P-Upk1b^tm1Pzg/J

Stock number: 010984
Strain synonyms: Upk1b^tm1Zhang
Research areas: Research Tools

Description

These targeted mutation mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos. This mutant mouse strain may be useful in studies of genitourinary system development.

Detailed description

These mutant mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.

This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

A targeting vector containing the Red Fluorescent Protein gene and duplicated loxP and FRT site flanked Kanamycin/neomycin selection cassettes was used to disrupt the exon containing the ATG translation start-site. The construct was electroporated into 129P2/OlaHsd derived E14Tg2a.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mutant mice were bred with B6129PF1/J mice (Stock No. 100492) for at least one generation to establish the colony.

Allele

Symbol: Upk1b^tm1Pzg
Name: targeted mutation 1, Pumin Zhang
MGI accession ID: MGI:4441453
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Upk1b
Marker name: uroplakin 1B
Chromosome: 16
Strain of origin: 129P2/OlaHsd
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos.
Molecular note: A targeting vector containing the Red Fluorescent Protein (RFP) gene followed, in reverse orientation, by two copies of a loxP and frt site-flanked kanamycin and neomycin resistance cassette (loxP-pA-frt-Kan&Neo-frt-loxP) was inserted into the first coding exon of the endogenous gene immediately following the ATG translation initiation codon. Red fluorescent signal is detected in the kidney and gonads of E15.5 heterozygous mutant embryos; fluorescence in the urogenital system mimics the expression pattern of the endogenous gene.