These targeted mutation mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos. This mutant mouse strain may be useful in studies of genitourinary system development.
Dr. Pumin Zhang, Baylor College of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Upk1b | uroplakin 1B |
These mutant mice express Red Fluorescent Protein from the endogenous Upk1b (uroplakin 1B) locus. Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 heterozygous embryos. Fluorescence mimics the endogenous expression pattern. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).
A targeting vector containing the Red Fluorescent Protein gene and duplicated loxP and FRT site flanked Kanamycin/neomycin selection cassettes was used to disrupt the exon containing the ATG translation start-site. The construct was electroporated into 129P2/OlaHsd derived E14Tg2a.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mutant mice were bred with B6129PF1/J mice (Stock No. 100492) for at least one generation to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos. |
Allele Name | targeted mutation 1, Pumin Zhang |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Upk1btm1Zhang; Upk1b-RFP |
Gene Symbol and Name | Upk1b, uroplakin 1B |
Gene Synonym(s) | |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Red fluorescence is detected in the bladder and gonads of the urogenital system from E15.5 embryos. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 16 |
Molecular Note | A targeting vector containing the Red Fluorescent Protein (RFP) gene followed, in reverse orientation, by two copies of a loxP and frt site-flanked kanamycin and neomycin resistance cassette (loxP-pA-frt-Kan&Neo-frt-loxP) was inserted into the first coding exon of the endogenous gene immediately following the ATG translation initiation codon. Red fluorescent signal is detected in the kidney and gonads of E15.5 heterozygous mutant embryos; fluorescence in the urogenital system mimics the expression pattern of the endogenous gene. |
When maintaining a live colony, these mice can be bred as heterozygotes. The Donating Investigator has not attempted to make the strain homozygous.
When using the B6;129P-Upk1btm1Pzg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010984 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Upk1b<tm1Zhang> |
Frozen Mouse Embryo | B6;129P-Upk1b<tm1Pzg>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Upk1b<tm1Pzg>/J | $2595.00 |
Frozen Mouse Embryo | B6;129P-Upk1b<tm1Pzg>/J | $3373.50 |
Frozen Mouse Embryo | B6;129P-Upk1b<tm1Pzg>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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