These transgenic mice express the Toxoplasma gondii uracil phosphoribosyltransferase (Uprt) gene fused to a hemagglutinin epitope tag driven by the chicken beta actin gene. Expression in brains has been demonstrated by Western blot. No overt phenotype has been reported by the donating laboratory.
Chris Q Doe, University of Oregon, HHMI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express the Toxoplasma gondii uracil phosphoribosyltransferase (Uprt) gene fused to a hemagglutinin epitope tag driven by the chicken beta actin gene. Expression in brains has been demonstrated by Western blot. No overt phenotype has been reported by the donating laboratory. Line 274 has a lower transgenic copy number than line 372 (see Stock No. 009668), but there are no overt phenotypic differences.
The chicken beta actin promoter drives expression of influenza hemagglutinin antigenic tag YPYDVPDYA fused to the Toxoplasma gondii-derived uracil phosphoribosyltransferase gene. The transgenic vector was introduced to B6D2F1 embryos. This line was crossed twice to C57BL/6 by the donating laboratory. This is line 274.
Expressed Gene | Uprt, uracil phosphoribosyltransferase, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 274, Chris Q Doe |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | pSTEC-CA:HAUPRT 274 |
Gene Symbol and Name | Tg(Actb-Uprt)274Cdoe, transgene insertion 274, Chris Q Doe |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | Uprt, uracil phosphoribosyltransferase, mouse, laboratory |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The chicken beta actin promoter drives expression of influenza hemagglutinin antigenic tag YPYDVPDYA fused to the Toxoplasma gondii-derived uracil phosphoribosyltransferase gene. The transgenic vector was introduced to B6D2F1 embryos. Line 274 was estabished. |
When maintained as a live colony, hemizygotes may be bred.
When using the B6;D2-Tg(Actb-Uprt)274Cdoe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010967 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(Actb-Uprt)274Cdoe |
Frozen Mouse Embryo | B6;D2-Tg(Actb-Uprt)274Cdoe/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(Actb-Uprt)274Cdoe/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;D2-Tg(Actb-Uprt)274Cdoe/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;D2-Tg(Actb-Uprt)274Cdoe/J Frozen Embryo | $3373.50 |
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