B6;D2-Tg(Actb-Uprt)274Cdoe/J

Stock number: 010967

Description

These transgenic mice express the Toxoplasma gondii uracil phosphoribosyltransferase (Uprt) gene fused to a hemagglutinin epitope tag driven by the chicken beta actin gene. Expression in brains has been demonstrated by Western blot. No overt phenotype has been reported by the donating laboratory.

Detailed description

These transgenic mice express the Toxoplasma gondii uracil phosphoribosyltransferase (Uprt) gene fused to a hemagglutinin epitope tag driven by the chicken beta actin gene. Expression in brains has been demonstrated by Western blot. No overt phenotype has been reported by the donating laboratory. Line 274 has a lower transgenic copy number than line 372 (see Stock No. 009668), but there are no overt phenotypic differences.

Development

The chicken beta actin promoter drives expression of influenza hemagglutinin antigenic tag YPYDVPDYA fused to the Toxoplasma gondii-derived uracil phosphoribosyltransferase gene. The transgenic vector was introduced to B6D2F1 embryos. This line was crossed twice to C57BL/6 by the donating laboratory. This is line 274.

Allele

Symbol: Tg(Actb-Uprt)274Cdoe
Name: transgene insertion 274, Chris Q Doe
MGI accession ID: MGI:4359807
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Actb-Uprt)274Cdoe
Marker name: transgene insertion 274, Chris Q Doe
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F1
Expressed genes: Uprt,uracil phosphoribosyltransferase,mouse, laboratory
Molecular note: The chicken beta actin promoter drives expression of influenza hemagglutinin antigenic tag YPYDVPDYA fused to the Toxoplasma gondii-derived uracil phosphoribosyltransferase gene. The transgenic vector was introduced to B6D2F1 embryos. Line 274 was estabished.