These mice carry a floxed allele of Grk6. When bred to mice with a cre recombinase gene, exons 3-9 of the targeted gene are deleted in the cre-expressing tissues of the offpring mice. This strain may be useful in studies of dopaminergic transmission, immune cell chemotaxis and colitis.
Robert Lefkowitz, Duke University Medical Center
These mice possess loxP sites on either side of exons 3-9 of Grk6 (G protein-coupled receptor kinase 6). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exons 3-9 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. The floxed allele appears to be fully functional and expresses normal levels of GRK6 protein.
Exons 3-9 were flanked by loxP sites and a loxP-neomycin resistance cassette was placed in intron 2. The mutation was created in 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Chimeras were crossed with C57BL/6 and resultant animals were crossed with a C57BL/6 background CMV-cre strain to remove the neomycin cassette leaving exons 3-9 flanked by loxP sites. This strain was backcrossed to C57BL/6 for 12 generations by the donating laboratory.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (5 of 5 markers segregating)in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
|Allele Name||targeted mutation 1, Marc G Caron|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Grk6, G protein-coupled receptor kinase 6|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||Exons 3 through 9 were flanked by an upstream floxed neomycin resistance cassette and a downstream single loxP site. Cre-mediated recombination in vivo under the control of a CMV promoter removed the neo cassette in the germline, leaving exons 3-9 flanked by loxP sites.|
|Mutations Made By|| |
Richard Premont, Duke University Medical Center
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S4-Grk6tm1Mca/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010962 in your Materials and Methods section.