B6.129S4-Grk5^tm2Rjl/J

Stock number: 010960
Research areas: Neurobiology Research, Research Tools

Description

These mice carry a floxed allele of Grk5. When bred to mice with a cre recombinase gene, exons 7 and 8 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. This strain may be useful in mechanistic studies of muscarinic receptors and Alzheimer's disease.

Detailed description

These mice possess loxP sites on either side of exons 7 and 8 of Grk5 (G protein-coupled receptor kinase 5). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exons 7 and 8 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. The floxed allele appears to be fully functional and expresses normal levels of GRK5 protein.

When bred to B6.FVB-Tg(Myh6-cre)2182Mds/J mice (Stock No. 011038) expressing Cre recombinase in cardiac muscle cells, offspring exhibit protection from cardiac hypertrophy following transverse aortic constriction.

Development

Exons 7 and 8 were flanked by loxP sites and a neomycin resistance cassette followed by a loxP site was placed in intron 8. The mutation was created in 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Transient transfection with cre recombinase excised the neomycin resistance cassette, leaving exons 7 and 8 floxed. This strain was backcrossed to C57BL/6 for 12 generations by the donating laboratory.

Allele

Symbol: Grk5^tm2Rjl
Name: targeted mutation 2, Robert J Lefkowitz
MGI accession ID: MGI:4359730
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: GRK5 flox
Marker symbol: Grk5
Marker name: G protein-coupled receptor kinase 5
Chromosome: 19
Strain of origin: 129S4/SvJaeSor
Molecular note: Exons 7 and 8 were flanked by loxP sites and a neomycin resistance cassette followed by a loxP site was placed in intron 8. Transient transfection with cre recombinase excised the neomycin resistance cassette, leaving exons 7 and 8 floxed.