These mice carry a floxed allele of Grk5. When bred to mice with a cre recombinase gene, exons 7 and 8 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. This strain may be useful in mechanistic studies of muscarinic receptors and Alzheimer's disease.
Robert Lefkowitz, Duke University Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Grk5 | G protein-coupled receptor kinase 5 |
These mice possess loxP sites on either side of exons 7 and 8 of Grk5 (G protein-coupled receptor kinase 5). Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exons 7 and 8 of the targeted gene are deleted in the cre-expressing tissues in the offspring mice. The floxed allele appears to be fully functional and expresses normal levels of GRK5 protein.
When bred to
B6.FVB-Tg(Myh6-cre)2182Mds/J mice (Stock No. 011038) expressing Cre recombinase in cardiac muscle cells, offspring exhibit protection from cardiac hypertrophy following transverse aortic constriction.
Exons 7 and 8 were flanked by loxP sites and a neomycin resistance cassette followed by a loxP site was placed in intron 8. The mutation was created in 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Transient transfection with cre recombinase excised the neomycin resistance cassette, leaving exons 7 and 8 floxed. This strain was backcrossed to C57BL/6 for 12 generations by the donating laboratory.
Allele Name | targeted mutation 2, Robert J Lefkowitz |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | GRK5 flox |
Gene Symbol and Name | Grk5, G protein-coupled receptor kinase 5 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 19 |
Molecular Note | Exons 7 and 8 were flanked by loxP sites and a neomycin resistance cassette followed by a loxP site was placed in intron 8. Transient transfection with cre recombinase excised the neomycin resistance cassette, leaving exons 7 and 8 floxed. |
Mutations Made By | Richard Premont, Duke University Medical Center |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S4-Grk5tm2Rjl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010960 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Grk5<tm2Rjl> |
Frozen Mouse Embryo | B6.129S4-Grk5<tm2Rjl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Grk5<tm2Rjl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Grk5<tm2Rjl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Grk5<tm2Rjl>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.