Targeted mutant mice have normal viability, fertility, balance and coordination. This strain may be useful in studying the role of G protein-coupled receptor kinases.
Robert Lefkowitz, Duke University Medical Center
Targeted mutant Grk4 (Grk4, G protein-coupled receptor kinase 4) mice have normal viability, fertility, balance and coordination. Expression is eliminated in testes, the primary site of expression.
A NsiI fragment containing exon 7 was replaced with a neomycin resistance cassette. The mutation was created in 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells and the strain was backcrossed more than 12 times to C57BL/6J by the donating laboratory.
|Allele Name||targeted mutation 1, Robert J Lefkowitz|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||GRK4 KO|
|Gene Symbol and Name||Grk4, G protein-coupled receptor kinase 4|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||A NsiI fragment containing exon 7 was replaced with a neomycin resistance cassette.|
|Mutations Made By|| |
Richard Premont, Duke University Medical Center
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6.129S4-Grk4tm1Rjl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010958 in your Materials and Methods section.