Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Screening of third generation mice (G3) on standard chow identified an 8 week old male (born 9/15/2004) with a 4 hour fasted plasma triglyceride level of 187 mg/dl, which is increased in comparison to controls. Retesting one week later confirmed this observation with a reading of 204 mg/dl. This mutant mouse strain may be useful in studies of triglyceride and lipid homeostasis.
This phenotypic deviant was identified following multidose ethylnitrosourea (ENU) treatments to induce mutations in male founder C57BL/6J mice (Stock No. 000664), in complex heart, lung, blood, and sleep disorders at the Mouse Heart, Lung, Blood, and Sleep Disorders (HLBS) Center at The Jackson Laboratory. The mutation has been identified as a C to T transition mutation at nucleotide position 1197 in the coding region of Krüppel-like Factor 15 (Klf15), resulting in a missense mutation at amino acid 340 in the encoded protein.
|Allele Name||heart, lung and blood, 444|
|Allele Type||Chemically induced (ENU) (Null/Knockout, Null/Knockout)|
|Gene Symbol and Name||Klf15, Kruppel-like factor 15hlb444, heart, lung and blood, 444|
|Strain of Origin||C57BL/6J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen. The causative mutation is a C to T transition mutation at nucleotide position 1197 in the coding region of KrÃ¼ppel-like Factor 15 (Klf15), resulting in a missense mutation at amino acid 340 in the encoded protein.|