Mice that are homozygous for the targeted mutation are viable, fertile and normal in size.  In contrast to the single S270H knockin, which exhibits increased GABA sensitivity and multiple behavioral and neurological phenotypes, the double S270H L277A mutation restores GABA sensitivity.

Western blot analysis indicates an increase in the amount of GABAR subunits alpha3, beta2 , gamma3 and a decrease in alpha1 and beta3. Homozygous mice exhibit an increase in motor activity, but no other overt phenotype.
Mutant mice show alterations in some, but not all, behavioral responses to alcohol and general anesthetics.


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This targeted mutation of gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 (<i>Gabra1</i>), gene displays hyperactivity and may be useful in the study of alcohol and general anesthetic action in the nervous system.

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