These suppressor of cytokine signaling 3 (Socs3)-floxed mice may be useful in generating conditional mutations to study cytokine signaling, inflammatory responses, energy homeostasis and diabetes.
Eleftheria Maratos-Flier, Beth Israel Deaconess Medical Center, Harvard Medical School
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cytokine signaling, inflammatory responses, energy homeostasis and diabetes.
For example, when crossed to a strain expressing Cre recombinase in the myeloid cell lineage (see Stock No. 004781), this mutant mouse strain may be useful in studies of cytokine signalling and inflammation.
When crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of leptin sensitivity and obesity.
When crossed to a strain expressing Cre recombinase in neuronal cells (see Stock No. 003966), this mutant mouse strain may be useful in studies of leptin sensitivity and obesity.
A targeting vector was designed to place loxP sites in intron 1 and the 3' untranslated region of exon 2 of the targeted gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric mice and crossed to C57BL/6. Heterozygotes were intercrossed to maintain the colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Akihiko Yoshimura|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Socs3fl; Socs3flox|
|Gene Symbol and Name||Socs3, suppressor of cytokine signaling 3|
|Strain of Origin||129S4/SvJae|
|Molecular Note||LoxP sites were inserted in intron 1 and in the 3' UTR of exon 2, thereby surrounding the coding sequence. Southern blot confirmed recombination.|
|Mutations Made By|| |
Akihiko Yoshimura, Kyushu Universtiy
While maintaining a live colony, these mice are bred as either heterozygotes or homozygotes.
When using the Socs3fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #010944 in your Materials and Methods section.