B6.Cg-Tg(ACTB-UPF1*R844C)581Hcd/J

Stock number: 010939
Research areas: Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

Human beta actin drives expression of the dominant negative R844C form of the human (UPF1)cDNA in these transgenic mice resulting in T cell-related defects.

Detailed description

Human beta actin drives expression of the dominant negative R844C form of the human (UPF1) (UPF1 regulator of nonsense transcripts homolog (yeast)) cDNA in these transgenic mice. The fetal thymus shows a decrease in the number of cells, reduced CD4/CD8 double positive thymocytes, diminished expression of TCRβ and increased expression of CD25, suggesting a defect in pre-TCR signalling. Fetal thymocytes also demonstrate diminished Vβ to DβJβ rearrangement.

Development

The human beta actin (ACTB) promoter drives expression of a dominant negative R844C mutant cDNA of the human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) gene. The mutation was introduced to B6129F1 embryos and the strain was backcrossed seven times to C57BL/6. This is founder line 581.

Allele

Symbol: Tg(ACTB-UPF1*R844C)581Hcd
Name: transgene insertion 581, Harry C Dietz
MGI accession ID: MGI:4820740
Generation method: Transgenic
Attributes: Dominant negative; Inserted expressed sequence; Humanized sequence
Synonyms: RNT1 Dom Neg
Marker symbol: Tg(ACTB-UPF1*R844C)581Hcd
Marker name: transgene insertion 581, Harry C Dietz
Chromosome: UN
Strain of origin: (C57BL/6 x 129)F1
Expressed genes: UPF1,UPF1, RNA helicase and ATPase,human
Molecular note: The human beta actin (ACTB) promoter drives expression of a dominant negative R844C mutant cDNA of the human UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1) gene. Line 581 was generated.