Hemizygous mice are viable and express mouse hepsin (<i>Hpn</i>) and humanized Renilla Green Fluorescent Protein under the control of the rat probasin promoter.   Tissues in the transgenic animals show GFP fluorescence patterns consistent with the hepsin transgene expression, specifically in the ventral lobe of the prostate. Immunofluorescence staining with antibodies specific to basement membrane, histological and electron microscopical examination suggest that the basement membrane in the prostate gland is severely compromised.  This mutant mouse strain may be useful in studies of prostate carcinogenesis and metastasis. <p> When crossed with mice carrying the LBP-Tag line 12T-7f, Tg(Pbsn-Tag)12T7fRjm transgene (Kasper S et al., Lab Invest. 1998 Jun;78(6):i-xv), 20% of the resulting double transgenic mice (at 21 weeks of age) develop prostate cancer metastasis in liver, lung and bone.

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This transgenic line expresses hepsin and hrGFP in the ventral lobe of the prostate and exhibits disruptions in the basement membrane in mature males. This mutant mouse strain may be useful in studies of prostate carcinogenesis and metastasis.

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