STOCK Mnx1^tm1Slp/J

Stock number: 010928
Strain synonyms: Hb9 FL
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring.

Detailed description

These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring. This strain may be useful in studies of neurodevelopment.

Development

Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2⁺ Tyr⁺ Kitl ⁺-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity.

Allele

Symbol: Mnx1^tm1Slp
Name: targeted mutation 1, Samuel L Pfaff
MGI accession ID: MGI:4359084
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mnx1
Marker name: motor neuron and pancreas homeobox 1
Chromosome: 5
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity.