These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring.
Samuel L Pfaff, The Salk Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mnx1 | motor neuron and pancreas homeobox 1 |
These mice possess loxP sites on either side of exon3 of the Mnx1 (motor neuron and pancreas homeobox 1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice with a cre recombinase gene, exon 3 is deleted in cre-expressing tissues in the offspring. This strain may be useful in studies of neurodevelopment.
Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity.
Allele Name | targeted mutation 1, Samuel L Pfaff |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Hb9 FL; Mnx1tm1Spf |
Gene Symbol and Name | Mnx1, motor neuron and pancreas homeobox 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 5 |
Molecular Note | Exon 3 of the targeted gene was flanked by loxP sites and a neomycin cassette flanked by FRT sites was introduced to intron 2. The targeting vector was electroporated into 129S1/Sv Oca2+ Tyr+ Kitl +-derived W9.5 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and then to CB6F1 background FLP mice to remove the neomycin cassette before the strain was bred to homozygosity. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Mnx1tm1Slp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010928 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Mnx1<tm1Spf> |
Frozen Mouse Embryo | STOCK Mnx1<tm1Slp>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mnx1<tm1Slp>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mnx1<tm1Slp>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Mnx1<tm1Slp>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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