These Slpi knock-out mice may be useful for studying wound healing, infectious diseases, and asthma.
Sharon M Wahl, National Institutes of Health
A neo cassette replaces nucleotide -849 through 46 of the secretory leukocyte peptidase inhibitor (Slpi) gene, abolishing gene expression. Homozygotes are viable and fertile. SLPI is expressed by epithelial and inflammatory cells at mucosal sites and in draining lymph nodes where it plays an important role in the control of local inflammation and infection. SLPI has been shown to reduce bacterial infiltrates and prevent the transmission of viruses, including HIV. SLPI knockout mice exhibit increased protease activity, aberrant innate and adaptive immune responses, and altered susceptibility to infection. They exhibit a delay in wound healing.
A targeting vector was designed to replace sequence from nucleotide -849 through 46 of the secretory leukocyte peptidase inhibitor (Slpi) gene with a neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to B6129SF1/J mice (Stock No. 101043) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Sharon M Wahl|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Slpi, secretory leukocyte peptidase inhibitor|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Sequence extending from nucleotide -849 through nucleotide 46 was replaced with a neomycin selection cassette. Deletion of this region eliminated regulatory elements, sequence encoding the first 16 amino acids, and introduced a frameshift mutation. Northern blot analysis confirmed the absence of transcript in homozygous mutant mice.|
|Mutations Made By|| |
Sharon Wahl, National Institutes of Health
When maintaining a live colony, homozygous mice may be bred together.
When using the SLPI-null mouse strain in a publication, please cite the originating article(s) and include JAX stock #010926 in your Materials and Methods section.