On the C57BL/6J background no homozygotes were found to survive to birth, but on the 129S1/SvImJ background (stock #010917) approximately 10% of homozygotes survive, have a diluted coat color, and may have a more severe electroconvulsive seizure phenotype than Szt2tm1Frk homozygotes. Heterozygotes have a slight increase in electroconvulsion over wild-type controls.
The 129P2/OlaHsd derived mutant ES cell clone XH662 from Bay Genomics was microinjected into blastocysts at The Jackson Laboratory and a resultant chimera was crossed to C57BL/6J and backcrossing continued until generation N10 and then the strain was maintained by sibling intercross until 2009 when heterozygous sperm were cryopreserved.
|Allele Name||gene trap XH662, BayGenomics|
|Allele Type||Gene trapped (Reporter, Null/Knockout)|
|Gene Symbol and Name||Szt2, seizure threshold 2|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||The gene trap vector was inserted in exon 21. Northern blot analysis indicates an increase in expression of the mutant transcript in whole brains from homozygous mice.|