These mice harbor a conditional knockout allele of the Flna gene. When bred to mice expressing cre recombinase, the floxed exons are deleted in the tissues expressing cre in the offspring. This strain may be useful in mechanistic studies of neuronal and cardiovascular development.
Christopher Walsh, Boston Children's Hospital
These mice possess loxP sites on either side of exons 2-7 of the Flna (filamin, alpha) gene. Mice that are homozygous/hemizygous for this X-linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice expressing cre recombinase, the floxed exons are deleted in the tissues expressing cre in the offspring.
When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of vascular development.
A loxP-flanked neomycin resistance cassette was placed in intron 2 and an additional loxP site was placed in intron 7 of the targeted gene using 129S4/SvJae-derived J1 embryonic stem (ES) cells. Transfection with a cre recombinase-expressing plasmid excised the neomycin cassette, leaving exons 3-7 flanked by loxP sites. This strain was maintained on a background incorporating 129, C57BL/6 and other unknown strains by the donating laboratory.
|Allele Name||targeted mutation 1.1, Christopher A Walsh|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Flnac; FlnAloxP|
|Gene Symbol and Name||Flna, filamin, alpha|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A combination of homologous recombination and transient cre expression resulted in the flanking of exons 3-7 with loxP sites.|
|Mutations Made By|| |
Yuanyi Feng, Northwestern University
When maintained as a live colony homozygous/hemizyous animals may be bred. The targeted gene is X-linked.
When using the STOCK Flnatm1.1Caw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010907 in your Materials and Methods section.