B6.Cg-Tg(Gfap-EGFP)3739Sart/J

Stock number: 010835
Product name: GFAP-GFP
Research areas: Research Tools, Sensorineural Research

Description

These Gfap-EGFP transgenic mice have EGFP expression directed primarily to Muller cells and may be useful in studies of retinal glial cells.

Detailed description

Mice harboring the Gfap-EGFP transgene are viable and fertile. The mouse glial fibrillary acidic protein (Gfap) promoter region directs expression of enhanced green fluorescent protein (EGFP) primarily to adult mouse retinal Muller cells. Developmental expression of EGFP mostly parallels GFAP in retinal astrocytes. Expression of EGFP first appears in Muller cells around two weeks of age. However, not all Muller cells express EGFP.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The Gfap-EGFP transgene was designed with a 2.5 kb 5' fragment of the mouse Gfap glial fibrillary acidic protein placed upstream of an enhanced green fluorescent protein (EGFP) coding sequence and SV40 polyA sequence. This transgene was microinjected into fertilized ICR(CD-1) mouse oocytes. Founder mice (line 3739) were mated with ICR(CD-1) mice for several generations and subsequently bred to C57BL/6 mice for 15 generations prior to arrival at The Jackson Laboratory. The Donating Investigator indicates that homozygotes are viable, but maintained the strain by mating hemizygotes to outbred/inbred mice. Upon arrival, transgenic mice were bred with C57BL/6J to establish the colony.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (5 of 5 markers segregating) in the development of the strain.

Allele

Symbol: Tg(Gfap-EGFP)3739Sart
Name: transgene insertion 3739, Vijay P Sarthy
MGI accession ID: MGI:4355151
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Gfap-EGFP)3739Sart
Marker name: transgene insertion 3739, Vijay P Sarthy
Chromosome: UN
Strain of origin: CD-1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Transgene expression is observed during retinal development, specifically in Muller cells.
Molecular note: A transgenic construct containing the EGFP gene under the control of a 2.5 kb 5' fragment of the mouse Gfap, glial fibrillary acidic protein, promoter was injected into fertilized ICR(CD-1) mouse eggs. Founder line 3747 was established and characterized. Line 3739 was also established but not described in the original reference. Transgene expression is observed during retinal development, specifically in Muller cells.