Mice that are homozygous for this targeted mutation of Per1, period homolog 1 (Drosophila), exhibit gradual circadian arrhythmicity when housed in constant darkness. This mutant mouse strain may be useful in studies of circadian rhythm and sleep pattern.
David R. Weaver, Univ of Massachusetts Medical School
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern or Western blot analysis of superchiasmatic nuclei. Homozygotes housed in constant darkness exhibit gradual circadian arrhythmicity (rhythmicity is not lost initially). Homozygotes exhibit phase shift responses to light. This mutant mouse strain may be useful in studies of circadian rhythm and sleep pattern.
A targeting vector containing PGKneo cassette was used to disrupt exons 2 through 12, which encode the translational start site, and the basic helix-loop-helix and PAS domains. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129S4/SvJae female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, mutant mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, David R Weaver|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||mPer1ldc; Per1 KO; Per1-; Per1m|
|Gene Symbol and Name||Per1, period circadian clock 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exons 2 through 12 were replaced with a neomycin selection cassette. The deleted region included the translational start site and the basic helix-loop-helix and PAS domains. Normal protein was undetected in homozygous mutant mice by immunohistochemical staining of superchiasmatic nuclei over a 24 hour period.|
|Mutations Made By|| |
Shin Yamazaki, Vanderbilt University
When maintaining a live colony, these mice can be bred as homozygotes.
When using the 129S-Per1tm1Drw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010831 in your Materials and Methods section.